Cytogenetic analyses of sequence-integrated clones
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Cytogenetic analyses of sequence-integrated clones
(a) Using FISH, fluorescent signals are observed at cytogenetic bands (grey) where fragments of a sequence-tagged bacterial artificial chromosome hybridize (red). (b) A clone selected on the basis of band location is used in FISH analysis to map the breakpoint of a translocation involving chromosomes 11 and 19 in a patient with multiple congenital malformations and mental retardation. The clone spans the breakpoint on chromosome 19; thus, the red signal is split between the derivative 11 and derivative 19 chromosomes and is present on the normal chromosome 19.
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Cytogeneticists can now go "FISH-ing" for chromosomal abnormalities, which are deletions and duplications that can cause disease. How exactly does FISH work?
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