An overview of miRNA biogenesis and function in Arabidopsis
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An overview of miRNA biogenesis and function in Arabidopsis
miRNAs are transcribed from a gene and processed by DCL1, SE and HYL1 into an RNA duplex (miRNA/miRNA*). The duplex is methylated by HEN and transported out of the nucleus by HST. The miRNA portion of the duplex binds AGO1 to form RISC. The miRNA bound in RISC base pairs with a target mRNA that is complementary to the miRNA. The target mRNA is repressed by either cleavage or translational inhibition.
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MicroRNAs interfere with mRNA translation, but they do so differently in plants and animals.
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