Original Article
Spinal Cord (2005) 43, 22–26. doi:10.1038/sj.sc.3101621; Published online 27 April 2004
Effectiveness of FK506 on lipid peroxidation in the spinal cord following experimental traumatic injury
Memduh Kaymaz1, Hakan Emmez1, Neslihan Bukan2, Ay
e Dursun3, Gökhan Kurt1, Hatice Paao
lu2 and Aydin Paaolu1
- 1Department of Neurosurgery, Gazi University Medical School, Beevler, Ankara, Turkey
- 2Department of Biochemistry, Gazi University Medical School, Beevler, Ankara, Turkey
- 3Department of Pathology, Gazi University Medical School, Beevler, Ankara, Turkey
Correspondence: Memduh Kaymaz, Hukukçu Dostlar Sitesi No:31, Çayyolu, 06530 Ankara, Turkey
Abstract
Study design:
An in vivo study in Wistar albino rats with injured spinal cord.
Setting
Department of Neurosurgery, Biochemistry and Pathology, Gazi University, Ankara, Turkey.
Objectives:
The aim of this study was to investigate and compare the effects of FK506 an immunosupressive agent with methylprednisolone (MP) on lipid peroxidation (LP) in injured spinal cord tissue.
Method:
A total of 28 adult healthy Wistar albino rats were subjected to traumatic spinal cord injuries (SCI) by using an aneurysmal clip compression technique, and they were divided into four groups. The G1 group (n=8) received FK506 (1 mg/kg); the G2 group (n=8) received FK506 (1 mg/kg) and MP (30 mg/kg); the G3 group (n=6) received only MP (30 mg/kg); and the G4 group (n=6) received no medication. The injured spinal cord tissue was studied by means of lipid peroxides, malondialdehyde (MDA), with thiobarbituric acid reaction and additionally the FK506 (G1); the MP (G3) groups were studied for histopathologic alterations 72 h after SCI with eight separate animals.
Results:
Although LP values of G1, G2, G3 showed no statistical difference between intergroup analyses (P=0.547), a histopathological examination revealed that in the group that received MP, the oedema pattern was more significant than the group that received FK506. Another interesting finding was the presence of polymorphonuclear leucocytes in the MP group, whereas no infiltration was found in the FK506 group.
Conclusion:
Analysis of the results indicated that FK506 is a valuable pharmacological agent that could be used to decrease the LP and polymorphonuclear leucocyte infiltration and inflamatory reactions in the injured spinal cord tissue.
Keywords:
spinal cord injury, FK-506, lipid peroxidation, methylprednisolone
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Spinal Cord Original Article
