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p19/Arf and p53 suppress sentinel lymph node lymphangiogenesis and carcinoma metastasis

Oncogene volume 27, pages 3145–3155 (2008)Cite this article

Abstract

The ability of tumor cells to metastasize is increasingly viewed as an interaction between the primary tumor and host tissues. Deletion of the p19/Arf or p53 tumor suppressor genes accelerates malignant progression and metastatic spread of 7,12-dimethylbenz(a)anthracene (DMBA)/12-O-tetradecanoyl-phorbol-13-acetate (TPA)-induced squamous cell carcinomas, providing a model system to address mechanisms of metastasis. Here, we show that benign pre-metastatic papillomas from wild-type mice trigger lymphangiogenesis within draining lymph nodes, whereas there is no growth of primary tumor lymphatic vessels. Lymph node lymphangiogenesis is greatly accelerated in papilloma-bearing p19/Arf- or p53-deficient mice, which coincides with the greater propensity of these tumors to progress to carcinomas and to metastasize. The extent of accumulation of B cells within the tumor-draining lymph nodes of wild-type mice predicted the level of lymph node lymphangiogenesis and metastatic potential. Arf or p53 deficiency strongly accelerated lymph node immune cell accumulation, in a manner that was associated with the extent of lymph node lymphatic sinus growth. This immune cell accumulation and lymph node lymphangiogenesis phenotype identifies host anti-tumor responses that could drive metastatic spread of cancers via the lymphatics.

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Acknowledgements

We thank Maria Harrell and Kay Gurley for advice, and NIH NCI R01-CA68328 (A Ruddell) and R01-CA99517 (CJ Kemp) grants for support.

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Authors and Affiliations

  1. Fred Hutchinson Cancer Research Center, Seattle, WA, USA

    A Ruddell, K S Kelly-Spratt, M Furuya, S S Parghi & C J Kemp

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  1. A Ruddell
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  2. K S Kelly-Spratt
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  3. M Furuya
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Correspondence to A Ruddell.

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Supplementary Information accompanies the paper on the Oncogene website (http://www.nature.com/onc).

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Ruddell, A., Kelly-Spratt, K., Furuya, M. et al. p19/Arf and p53 suppress sentinel lymph node lymphangiogenesis and carcinoma metastasis. Oncogene 27, 3145–3155 (2008). https://doi.org/10.1038/sj.onc.1210973

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