Oncogene

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Cigarette smoke induces demethylation of prometastatic oncogene synuclein-bold italic gamma in lung cancer cells by downregulation of DNMT3B

H Liu, Y Zhou, S E Boggs, S A Belinsky and J Liu

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Figure 2.

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Dose- and time-dependent induction of DNA demethylation and reexpression of SNCG in A549 cells by CSE. A549 cells were exposed to the indicated concentrations of CSE for 3 days or to a concentration of 2.5% CSE for different days. The medium was changed every day with fresh medium and CSE. By the end of treatment, genomic DNA and total RNA were isolated from untreated and treated cells. (a) Dose-dependent effects of CSE on SNCG mRNA expression and CpG island demethylation in A549 cells. (b) Time-dependent effects of CSE on CpG island demethylation in A549 cells examined by MSP (left panel). The methylation status of A549 cells treated with CSE for 3 days was further determined by genomic sequencing. (c) Kinetics of induction of SNCG mRNA expression by CSE in A549 cells. (d) Dose-dependent effects of NNK on SNCG mRNA expression in A549 cells.

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