FIGURE 4

Minimum common region of homozygous loss at 2q13 and expression of BIM. (a) Schematic illustration of BAC438K19, the BIM gene exons, and loss patterns of three cell lines (SP-53, Z-138 and Jeko-1). Gray boxes: exons (open reading frames) of BIM EL and BIM L. The open reading frame of BIM EL (597 bp) consists of three exons: exon 1 from 75 082 to 75 475 bp (394 bp) including the initiating codon (ATG), exon 2 from 49 074 to 49 177 bp (104 bp), and exon 3 from 34 990 to 35 088 bp (99 bp) including the termination codon (TGA), all on BAC438K19. Black and white circles: probes used for Southern blot analyses. Broken horizontal lines with white circles: homozygous loss (bands negative). Thick horizontal lines with black circles: no homozygous loss (bands positive). Thin horizontal lines: not confirmed whether homozygous loss or not. Bold broken horizontal arrows between probes 2 and 3 indicate the minimum common region of homozygous loss of 2q13. (b) Southern blot analyses using probes 1−6 for genomic DNAs of MCL cell lines. Lane 1, human placenta; lane 2, SP-53; lane 3, Granta 519 (G519); lane 4, Z-138; lane 5, REC-1; lane 6, NCEB-1; lane 7, Jeko-1; lane 8, JVM2. Bands of probe 1: human placenta (+), SP-53 (-), Granta 519 (+), Z-138 (+), REC-1 (+), NCEB-1 (+), Jeko-1 (-), and JVM2 (+). Bands of probes 2 and 3: human placenta (+), SP-53 (-), Granta 519 (+), Z-138 (-), REC-1 (+), NCEB-1 (+), Jeko-1 (-), and JVM2 (+). Bands of probe 4: human placenta (+), SP-53 (-), Granta 519 (+), Z-138 (-), REC-1 (+), NCEB-1 (+), Jeko-1 (+/-), and JVM2 (+). Bands of probes 5 and 6: human placenta (+), SP-53 (-), Granta 519 (+), Z-138 (-), REC-1 (+), NCEB-1 (+), Jeko-1 (+), and JVM2 (+). 'Control' indicates the representative control band of probe 3 (TCR probe) located under the bands of probe 6. (c) Northern blot analysis of BIM with seven MCL cell lines, B-cell lymphoma (Karpas 231) and Burkitt's lymphoma (Raji) cell lines. Control is -actin