Original Paper
Oncogene (2005) 24, 1375–1384. doi:10.1038/sj.onc.1208301 Published online 20 December 2004 doi:1208301a/sj.bjp.0704832
Myb proteins regulate the expression of diverse target genes
Georgina Lang1, Jonathan R White1,2, Mary J G Argent-Katwala1,3, Christopher G Allinson1 and Kathleen Weston1
1CRUK Centre for Cell and Molecular Biology, Institute of Cancer Research, 237 Fulham Road, London SW3 6JB, UK
Correspondence: K Weston, E-mail: kathy.weston@icr.ac.uk
2Current address: OSI Pharmaceuticals, Inc., Watlington Road, Oxford OX4 6LT, UK
3Current address: Decision Resources International, 50-51 Russell Square, London WC1B 4HQ, UK
Received 30 July 2004; Revised 1 October 2004; Accepted 20 October 2004; Published online 20 December 2004.
Abstract
Hematopoiesis, the process by which mature blood cells arise, is controlled by multiple transcription factors, which act in stage- and lineage-specific complexes. It is a major goal to elucidate the genes regulated by these transcription factors, in order to obtain a full understanding of the process and its malignant counterpart, leukemia. Myb family transcription factors play a central role in hematopoiesis. To identify new Myb family target genes, we have used an inducible dominant-negative protein for a subtraction cloning protocol in a model cell system (FDCP-Mix) with many characteristics of normal hematopoiesis. We present here a novel group of 29 validated Myb family target genes of diverse functions.
Keywords:
c-Myb, B-Myb, transcription, hematopoiesis, T cell
