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Absence of mutation in the putative tumor-suppressor gene KLF6 in colorectal cancers

Oncogene volume 24, pages 7253–7256 (2005)Cite this article

Abstract

The KLF6 gene encodes the Krüppel-like factor 6, a transcription factor that has been individualized as a tumor-suppressor gene involved in the regulation of cell proliferation and differentiation. Recently, high frequency (42%) of KLF6 mutations have been reported in colorectal cancers (CRC) as in prostate cancers, astrocytic gliomas and hepatocellular carcinomas. The aims of the study was to confirm the frequency of KLF6 mutations in a larger series of CRC than that previously published by using DNA extracted from frozen tissue samples, which have been proved to generate less mutational artefact than that extracted from formalin-fixed paraffin-embedded tissue samples, in order to compare KLF6 mutation frequency with that of other common genetic alterations and to determine genotype–phenotype correlations. Amplification and direct sequencing of KLF6 exon 2 of 76 CRC and matched normal frozen tissues was performed. Polymorphisms were observed in 14 cases, among which two (T35T and S116S) had not already been reported. No KLF6 somatic mutation was observed. Our data suggest a minor role of KLF6 mutation in colorectal carcinogenesis and underline the fact that the validity of sequence informations obtained from DNA extracted from formalin-fixed tissues may be limited.

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Acknowledgements

Le Ligue Nationale de Lutte Contre le Cancer.

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Authors and Affiliations

  1. INSERM U490 Laboratoire de Toxicologie Moléculairei, Université René Descartes, 45 rue des Saints-Pères, Paris, 75006, France

    Astrid Lièvre, Jean-François Côté, Nicolas Veyrie & Pierre Laurent-Puig

  2. Assistance Publique Hôpitaux de Paris, Hôpital Européen Georges Pompidou, service de gastroentérologie, 20 rue Leblanc, Paris, 75015, France

    Bruno Landi

  3. INSERM U674 Génomique des tumeurs solides, 27 rue Juliette Dodu, Paris, 75010, France

    Jessica Zucman-Rossi

  4. Assistance Publique Hôpitaux de Paris, Hôpital Européen Georges Pompidou, service de chirurgie générale digestive et oncologique, 20 rue Leblanc, Paris, 75015, France

    Anne Berger

  5. Assistance Publique Hôpitaux de Paris, Hôpital Européen Georges Pompidou, service de Biochimie, 20 rue Leblanc, Paris, 75015, France

    Pierre Laurent-Puig

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  1. Astrid Lièvre
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Correspondence to Pierre Laurent-Puig.

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Lièvre, A., Landi, B., Côté, JF. et al. Absence of mutation in the putative tumor-suppressor gene KLF6 in colorectal cancers. Oncogene 24, 7253–7256 (2005). https://doi.org/10.1038/sj.onc.1208867

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