FIGURE 5
FROM:
Immortalized fibroblasts from NF-
B RelA knockout mice show phenotypic heterogeneity and maintain increased sensitivity to tumor necrosis factor 
after transformation by v-Ras
Maria-Emily R Gapuzan, Oliver Schmah, Ashley D Pollock, Alexander Hoffmann and Thomas D Gilmore
BACK TO ARTICLEFigure 5.
Transforming activity of v-Ha-Ras in cell lines missing RelA, p50 (nfkb1 gene), or c-Rel. (a) Soft agar colony formation by v-Ras-transformed fibroblasts. The indicated cell types were infected with the indicated virus stocks, and were then plated in soft agar as described in Materials and methods. Values are relative to the number of colonies seen with v-Ras-infected wild-type 3T3 cells (100) and are the averages (with standard errors) of five independent assays with duplicate samples. c, uninfected cells; -, pBabe-infected cells; +, pBabe-v-Ras-infected cells. (b) Expression of v-Ha-Ras in Ras-transformed cell lines deficient in RelA, p50, or c-Rel. Extracts were prepared from pools of the indicated cells infected with a v-Ha-Ras virus or an empty virus (pBabe). Equal amounts of protein in cell extracts were analysed by Western blotting using antibodies specific for v-Ha-Ras and actin
