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  • Original Paper
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KIF14 is a candidate oncogene in the 1q minimal region of genomic gain in multiple cancers

Oncogene volume 24, pages 4741–4753 (2005)Cite this article

Abstract

Gain of chromosome 1q31–1q32 is seen in >50% of retinoblastoma and is common in other tumors. To define the minimal 1q region of gain, we determined genomic copy number by quantitative multiplex PCR of 14 sequence tagged sites (STSs) spanning 1q25.3–1q41. The most frequently gained STS at 1q32.1 (71%; 39 of 55 retinoblastoma) defined a 3.06 Mbp minimal region of gain between flanking markers, containing 14 genes. Of these, only KIF14, a putative chromokinesin, was overexpressed in various cancers by real-time RT–PCR. KIF14 mRNA was expressed in 20/22 retinoblastoma samples 100–1000-fold higher than in retina (t-test P=0.00002); cell lines (n=10) had higher levels than tumors (n=12) (P=0.009). KIF14 protein was overexpressed in retinoblastoma tumors and breast cancer cell lines by immunoblot. KIF14 was expressed in 4/4 breast cancer cell lines 31–92-fold higher than in normal breast tissue, in 5/5 medulloblastoma cell lines 22–79-fold higher than in fetal brain, and in 10/22 primary lung tumors 3–34-fold higher than in normal lung. Patients with lung tumors that overexpress KIF14 showed a trend toward decreased survival. KIF14 may thus be important in oncogenesis, and has promise as a prognostic indicator and therapeutic target.

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Acknowledgements

We thank S Pajovic and all members of the Gallie Laboratory for insightful input, Lisa Wang for statistical assistance, Solutions by Sequence Inc. (Toronto Western Hospital) for the use of equipment for QM-PCR, and D Bigner (Duke University), S Done, M Minden, and C Paige (OCI) for cell lines. This work was supported by grants to BLG from the National Cancer Institute of Canada with funds from the Terry Fox Run and the Canadian Cancer Society, the Canadian Genetic Diseases Network and the Canadian Institutes for Health Research, and by the Keene Retinoblastoma Perennial Plant Sale. TWC was supported by a Canadian Institutes of Health Research Canada Graduate Scholarship, a Natural Sciences and Engineering Research Council Postgraduate Scholarship, and a University of Toronto Vision Science Research Program Studentship.

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Authors and Affiliations

  1. Division of Cancer Informatics, Ontario Cancer Institute/Princess Margaret Hospital, University Health Network, Toronto, M5G 2M9, ON, Canada

    Timothy W Corson & Brenda L Gallie

  2. Department of Molecular & Medical Genetics, University of Toronto, Toronto, M5S 1A8, ON, Canada

    Timothy W Corson & Brenda L Gallie

  3. Labatt Brain Tumour Research Centre, Cancer Research Program, Hospital for Sick Children, Toronto, M5G 1X8, ON, Canada

    Annie Huang

  4. Division of Cellular & Molecular Biology, Ontario Cancer Institute/Princess Margaret Hospital, University Health Network, Toronto, M5G 2M9, ON, Canada

    Ming-Sound Tsao

  5. Department of Medical Biophysics, University of Toronto, Toronto, M5G 2M9, ON, Canada

    Ming-Sound Tsao & Brenda L Gallie

  6. Department of Laboratory Medicine and Pathobiology, University of Toronto, Toronto, M5G 1L5, ON, Canada

    Ming-Sound Tsao

  7. Department of Ophthalmology, University of Toronto, Toronto, M5G 1X5, ON, Canada

    Brenda L Gallie

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  1. Timothy W Corson
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Correspondence to Brenda L Gallie.

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Corson, T., Huang, A., Tsao, MS. et al. KIF14 is a candidate oncogene in the 1q minimal region of genomic gain in multiple cancers. Oncogene 24, 4741–4753 (2005). https://doi.org/10.1038/sj.onc.1208641

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