Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • Original Paper
  • Published:

Junctional recruitment of mammalian Scribble relies on E-cadherin engagement

Oncogene volume 24, pages 4330–4339 (2005)Cite this article

Abstract

Members of the LAP protein family, LET-413 in Caenorhabditis elegans, Scribble in Drosophila melanogaster and Erbin, Lano, Densin-180 and hScrib in mammals, have conserved structural features. LET-413 and Scribble are junctional proteins involved in establishing and maintaining epithelial cell polarity. scribble also behaves as a neoplastic tumor suppressor gene. We show here that, in epithelial cells, hScrib is recruited at cell–cell junctions in an E-cadherin-dependent manner as shown by calcium switch assays in MDCK cells, re-expression of E-cadherin in MDA-231 cells treated by 5-Aza-2′-deoxycytidine (5Aza) and siRNA experiments. hScrib is restricted at the basolateral membrane of epithelial cells by its LRR domain and is enriched in Triton X-100-insoluble fractions. In breast cancers, most lobular tumors did not express hScrib and E-cadherin while ductal tumors had a less frequent downregulation of hScrib. Our data provide additional insights on the modalities of recruitment of hScrib at the cell–cell junctions and establish a potential link between the E-cadherin and hScrib tumor suppressors.

This is a preview of subscription content, access via your institution

Access options

Buy this article

  • Purchase on Springer Link
  • Instant access to full article PDF
Buy now

Prices may be subject to local taxes which are calculated during checkout

Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Figure 6
Figure 7
Figure 8
Figure 9

Similar content being viewed by others

References

  • Albertson R, Chabu C, Sheehan A and Doe CQ . (2004). J. Cell Sci., 117, 6061–6070.

  • Audebert S, Navarro C, Nourry C, Chasserot-Golaz S, Lecine P, Bellaiche Y, Dupont JL, Premont RT, Sempere C, Strub JM, Van DA, Vitale N and Borg JP . (2004). Curr. Biol., 14, 987–995.

  • Baas AF, Smit L and Clevers H . (2004). Trends Cell Biol., 14, 312–319.

  • Benton R and St Johnston D . (2003). Curr. Biol., 13, 1330–1334.

  • Bertucci F, Borie N, Ginestier C, Groulet A, Charafe-Jauffret E, Adelaide J, Geneix J, Bachelart L, Finetti P, Koki A, Hermitte F, Hassoun J, Debono S, Viens P, Fert V, Jacquemier J and Birnbaum D . (2004). Oncogene, 23, 2564–2575.

  • Berx G and Van Roy F . (2003). Breast Cancer Res., 3, 289–293.

  • Bilder D . (2004). Genes Dev., 18, 1909–1925.

  • Bilder D and Perrimon N . (2000). Nature, 403, 676–680.

  • Borg JP, Marchetto S, Le Bivic A, Ollendorff V, Jaulin-Bastard F, Saito H, Fournier E, Adelaide J, Margolis B and Birnbaum D . (2000). Nat. Cell Biol., 2, 407–414.

  • Dow LE, Brumby AM, Muratore R, Coombe ML, Sedelies KA, Trapani JA, Russell SM, Richardson HE and Humbert PO . (2003). Oncogene, 22, 9225–9230.

  • Droufakou S, Deshmane V, Roylance R, Hanby A, Tomlinson I and Hart IR . (2001). Int. J. Cancer, 92, 404–408.

  • Ginestier C, Charafe-Jauffret E, Bertucci F, Eisinger F, Geneix J, Bechlian D, Conte N, Adelaide J, Toiron Y, Nguyen C, Viens P, Mozziconacci MJ, Houlgatte R, Birnbaum D and Jacquemier J . (2002). Am. J. Pathol., 161, 1223–1233.

  • Graff JR, Herman JG, Lapidus RG, Chopra H, Xu R, Jarrard DF, Isaacs WB, Pitha PM, Davidson NE and Baylin SB . (1995). Cancer Res., 55, 5195–5199.

  • Grindstaff KK, Yeaman C, Anandasabapathy N, Hsu SC, Rodriguez-Boulan E, Scheller RH and Nelson WJ . (1998). Cell, 93, 731–740.

  • Huang YZ, Wang Q, Xiong WC and Mei L . (2001). J. Biol. Chem., 276, 19318–19326.

  • Jaulin-Bastard F, Arsanto JP, Le Bivic A, Navarro C, Vely F, Saito H, Marchetto S, Hatzfeld M, Santoni MJ, Birnbaum D and Borg JP . (2002). J. Biol. Chem., 277, 2869–2875.

  • Lallemand D, Curto M, Saotome I, Giovannini M and McClatchey AI . (2003). Genes Dev., 17, 1090–1100.

  • Laprise P, Viel A and Rivard N . (2004). J. Biol. Chem., 279, 10157–10166.

  • Lee S, Fan S, Makarova O, Straight S and Margolis B . (2002). Mol. Cell Biol., 22, 1778–1791.

  • Legouis R, Gansmuller A, Sookhareea S, Bosher JM, Baillie DL and Labouesse M . (2000). Nat. Cell Biol., 2, 415–422.

  • Legouis R, Jaulin-Bastard F, Schott S, Navarro C, Borg JP and Labouesse M . (2003). EMBO Rep., 4, 1096–1102.

  • Mantovani F, Massimi P and Banks L . (2001). J. Cell Sci., 114, 4285–4292.

  • Mathew D, Gramates LS, Packard M, Thomas U, Bilder D, Perrimon N, Gorczyca M and Budnik V . (2002). Curr. Biol., 12, 531–539.

  • Mizuno K, Suzuki A, Hirose T, Kitamura K, Kutsuzawa K, Futaki M, Amano Y and Ohno S . (2003). J. Biol. Chem., 278, 31240–31250.

  • Musch A, Cohen D, Yeaman C, Nelson WJ, Rodriguez-Boulan E and Brennwald PJ . (2002). Mol. Biol. Cell, 13, 158–168.

  • Nakagawa S and Huibregtse JM . (2000). Mol. Cell. Biol., 20, 8244–8253.

  • Nakagawa S, Yano T, Nakagawa K, Takizawa S, Suzuki Y, Yasugi T, Huibregtse JM and Taketani Y . (2004). Br. J. Cancer, 90, 194–199.

  • Nelson WJ . (2003). Nature, 422, 766–774.

  • Nourry C, Grant SG and Borg JP . (2003). Sci. STKE, 179, RE7.

  • Ohno H, Hirabayashi S, Iizuka T, Ohnishi H, Fujita T and Hata Y . (2002). Oncogene, 21, 7042–7049.

  • Roche JP, Packard MC, Moeckel-Cole S and Budnik V . (2002). J. Neurosci., 22, 6471–6479.

  • Santoni MJ, Pontarotti P, Birnbaum D and Borg JP . (2002). Trends Genet., 18, 494–497.

  • Strathdee G . (2002). Semin. Cancer Biol., 12, 373–379.

  • Vasioukhin V, Bauer C, Degenstein L, Wise B and Fuchs E . (2001). Cell, 104, 605–617.

  • Walikonis RS, Oguni A, Khorosheva EM, Jeng CJ, Asuncion FJ and Kennedy MB . (2001). J. Neurosci., 21, 423–433.

  • Zeitler J, Hsu CP, Dionne H and Bilder D . (2004). J. Cell Biol., 167, 1137–1146.

Download references

Acknowledgements

We are grateful to Dr T Nagase for the KIAA0147 cDNA, Dr R Kemler for E-cadherin-positive and -negative fibroblasts, Dr G Monges for human colon samples and R Galindo for cell sorting expertises. The Laboratory of Molecular Pharmacology is supported by INSERM, Institut Paoli-Calmettes, Ligue Nationale Contre le Cancer (Label Ligue), Fondation de France, Cancéropole PACA and ACI ‘Jeune Chercheur’. CN and SA are recipients of Conseil Régional/Ipsogen fellowships. SN is a recipient of a Ligue Contre le Cancer fellowship.

Author information

Author notes

  1. Supplementary Information accompanies the paper on the Oncogene website (http://www.nature.com/onc)

Authors and Affiliations

  1. Marseille Cancer Institute, Molecular Pharmacology, UMR 599 Inserm-Institut Paoli-Calmettes, 27 boulevard Leï Roure, 13009, Marseille, France

    Christel Navarro, Sébastien Nola, Stéphane Audebert, Marie-Josée Santoni, Sylvie Marchetto & Jean-Paul Borg

  2. NMDA, Faculté des Sciences de Luminy, case 907, 13288, Marseille, cedex 09, France

    Jean-Pierre Arsanto & André Le Bivic

  3. Molecular Oncology, UMR 599 Inserm-Institut Paoli-Calmettes, France

    Christophe Ginestier, Jocelyne Jacquemier & Daniel Birnbaum

  4. Imagerie Cellulaire, UMR 599 Inserm-Institut Paoli-Calmettes, France

    Daniel Isnardon

Authors
  1. Christel Navarro
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Sébastien Nola
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Stéphane Audebert
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Marie-Josée Santoni
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Jean-Pierre Arsanto
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Christophe Ginestier
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Sylvie Marchetto
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Jocelyne Jacquemier
    View author publications

    You can also search for this author in PubMed Google Scholar

  9. Daniel Isnardon
    View author publications

    You can also search for this author in PubMed Google Scholar

  10. André Le Bivic
    View author publications

    You can also search for this author in PubMed Google Scholar

  11. Daniel Birnbaum
    View author publications

    You can also search for this author in PubMed Google Scholar

  12. Jean-Paul Borg
    View author publications

    You can also search for this author in PubMed Google Scholar

Electronic supplementary material

Rights and permissions

Reprints and permissions

About this article

Cite this article

Navarro, C., Nola, S., Audebert, S. et al. Junctional recruitment of mammalian Scribble relies on E-cadherin engagement. Oncogene 24, 4330–4339 (2005). https://doi.org/10.1038/sj.onc.1208632

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1038/sj.onc.1208632

Keywords

This article is cited by

Search

Advanced search

Quick links