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Defining minimum genomic regions of imbalance involved in testicular germ cell tumors of adolescents and adults through genome wide microarray analysis of cDNA clones

Oncogene volume 23, pages 9142–9147 (2004)Cite this article

Abstract

Identifying changes in DNA copy number can pinpoint genes that may be involved in tumor development. Here we have defined the smallest overlapping regions of imbalance (SORI) in testicular germ cell tumors other than the 12p region, which has been previously investigated. Definition of the regions was achieved through comparative genomic hybridization (CGH) analysis of a 4559 cDNA clone microarray. A total of 14 SORI were identified, which involved at least five of the 11 samples analysed. Many of these refined regions were previously reported using chromosomal or allelic imbalance studies. The SORI included gain of material from the regions 4q12, 17q21.3, 22q11.23 and Xq22, and loss from 5q33, 11q12.1, 16q22.3 and 22q11. Comparison with parallel chromosomal CGH data supported involvement of most regions. The various SORI span between one and 20 genes and highlight potential oncogenes/tumor suppressor genes to be investigated further (Supplementary material is available at http://www.crukdmf.icr.ac.uk/array/array.html).

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Acknowledgements

We thank the Lance Armstrong Foundation, Cancer Research UK and the Dutch Cancer Society (including Grant DDHK 97-1685) for their support.

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Authors and Affiliations

  1. Molecular Cytogenetics, Section of Molecular Carcinogenesis, Institute of Cancer Research, Sutton, Surrey, UK

    Alan McIntyre, Brenda Summersgill, Osman Jafer, Sandrine Rodriguez & Janet Shipley

  2. Department of Pathology, Erasmus MC-University Medical Center Rotterdam, Daniel den Hoed Cancer Center, Josephine Nefkens Institute, Rotterdam, 3000 DR, The Netherlands

    Gaetano Zafarana, J Wolter Oosterhuis, Ad JM Gillis & Leendert Looijenga

  3. Cell Transformation, Section of Molecular Carcinogenesis, Institute of Cancer Research, Sutton, Surrey, UK

    Colin Cooper & Jeremy Clark

  4. Academic Department of Urology, The Royal Marsden National Health Service Trust and Institute of Cancer Research, Sutton, Surrey, UK

    Robert Huddart

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  1. Alan McIntyre
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Correspondence to Janet Shipley.

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McIntyre, A., Summersgill, B., Jafer, O. et al. Defining minimum genomic regions of imbalance involved in testicular germ cell tumors of adolescents and adults through genome wide microarray analysis of cDNA clones. Oncogene 23, 9142–9147 (2004). https://doi.org/10.1038/sj.onc.1208115

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