FIGURE 5
FROM:
-parvin inhibits integrin-linked kinase signaling and is downregulated in breast cancer
Perry S Mongroo, Cameron N Johnstone, Izabela Naruszewicz, Chungyee Leung-Hagesteijn, Raphael K Sung, Leanne Carnio, Anil K Rustgi and Gregory E Hannigan
BACK TO ARTICLEFigure 5.
ParvB protein expression is downregulated in human breast tumors and breast cancer cell lines. Whole-cell lysates (50 
g/lane) of (a) human cell lines and (b) paired normal and tumor breast tissue lysates were subjected to 12% SDS–PAGE and immunoblotted with ParvB antibody. Blots were then sequentially stripped and reprobed with ILK and GAPDH antibodies. Values represent ratios of tumor to normal ParvB levels, determined by densitometry of ParvB doublet bands, each internally normalized against the GAPDH signal (see Materials and methods). (c) Normal and tumor breast tissue lysates (700 g each) were analysed in an ILK immune complex kinase assay using MBP as exogenous substrate. (d) A total of seven patient-matched tumor/normal mammary gland samples were analysed for ParvB protein levels. Results are presented as relative levels for each patient sample, normalized to GAPDH expression, by densitometry. The three left-most samples are derived from the blots in (b)
