FIGURE 2
FROM:
Mutation of GATA3 in human breast tumors
Jerry Usary, Victor Llaca, Gamze Karaca, Shafaq Presswala, Mehmet Karaca, Xiaping He, Anita Langerød, Rolf Kåresen, Daniel S Oh, Lynn G Dressler, Per E Lønning, Robert L Strausberg, Stephen Chanock, Anne-Lise Børresen-Dale and Charles M Perou
BACK TO ARTICLEFigure 2.
Western blot analysis of GATA3 protein expression. In all, 11 breast tumors, one normal breast sample and the MCF-7 cell line protein extracts were run on a 4–20% SDS–PAGE gel and assayed for expression of GATA3 protein using the sc-269 monoclonal antibody. The expected wild-type band of 48 kDa was seen in most lanes along with a smaller approximately 23 kDa band, while tumor BR99-0348 (identified by asterix), BR99-0207 and the MCF-7 cell line showed their predicted truncated GATA3 protein products. On the right is a shorter exposure of BR99-0207 and MCF-7 to show finer detail because their expression levels were high compared to the other samples. On the far right is a Western blot of the same normal breast sample using a second monoclonal antibody (sc-268), which also identified both the 48 and 23 kDa bands
