Abstract
The precise temporal and spatial emergence of hematopoietic stem cells (HSC) in the murine embryo has been somewhat controversial largely due to differences in the assays utilized to demonstrate HSC repopulating ability. One strategy is to determine where and when one can first detect HSC that engraft upon transplantation into lethally irradiated adult mice. However, knowing that the primary sites and patterns of hematopoiesis change during ontogeny, an alternative strategy is to select transplantation models where the recipient subjects more closely mirror the stage of development of the donor cells. In this regard, perhaps the most relevant assay to determine the presence of HSC activity in the early embryo is to transplant the donor cells in utero into recipient embryos. Other recipient models that may permit engraftment of embryonic cells include the use of submyeloablated or genetically HSC deficient newborn mice. Additional informative strategies have included co-culturing embryonic tissues that appear to lack HSC activity, with stromal cells derived from different developmental sites of hematopoiesis to induce HSC emergence, followed by transplantation as a means to determine which embryonic tissues possess HSC potential. This review will highlight some of the various transplantation assays used to identify HSC from embryonic tissues.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 50 print issues and online access
$259.00 per year
only $5.18 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Cumano A, Ferraz JC, Klaine M, Di Santo JP and Godin I . (2001). Immunity, 15, 477–485.
de Bruijn M, Ma X, Robin C, Ottersbach K, Sanchez M-J and Dzierzak E . (2002). Immunity, 16, 673–683.
Godin I, Garcia-Porrero JA, Dieterlen-Lievre F and Cumano A . (1999). J. Exp. Med., 190, 43–52.
Johnson SA and Yoder MC . (in press). Developmental Hematopoiesis. Baron MH (ed). Humana Press: Totowa, NJ.
Kumaravelu P, Hook L, Morrison A, Ure J, Zhal S, Zuayev S, Ansell J and Medvinsky A . (2002). Development, 129, 4891–4899.
Matsuoka S, Tsuji K, Hisakawa H, Xu M-J, Ebihara Y, Ishii T, Sugiyama D, Manabe A, Tanaka R, Ikeda Y, Asano S and Nakahata T . (2001). Blood, 98, 6–12.
McGrath K, Koniski A, Malik J and Palis J . (2003). Blood, 101, 1669–1676.
Muller A, Medvinsky A, Strouboulis J, Grosveld F and Dzierzak E . (1994). Immunity, 1, 291–301.
North T, de Bruijn M, Stacy T, Talebian L, Lind E, Robin C, Binder M, Dzierak E and Speck N . (2002). Immunity, 16, 661–672.
Orlic D and Bodine D . (1994). Blood, 84, 3991–3994.
Palis J, Chan RJ, Koniski A, Patel R, Starr M and Yoder MC . (2001). Proc. Natl. Acad. Sci. USA, 98, 4528–4533.
Palis J, Robertson S, Kennedy M, Wall C and Keller G . (1999). Development, 126, 5073–5081.
Palis J and Yoder M . (2001). Exp. Hematol., 29, 927–936.
Ramshaw H, Crittenden R, Dooner M, Peters S, Rao S and Quesenberry P . (1995). Biol. Blood Marrow Transplant., 1, 74–80.
Rao S, Peters S, Crittenden R, Stewart F, Ramshaw H and Quesenberry P . (1997). Exp. Hematol., 25, 114–121.
Stewart F, Crittenden R, Lowry P, Pearson-White S and Quesenberry P . (1993). Blood, 81, 2566–2571.
Toles JF, Chui DHK, Belbeck LW, Starr E and Barker JE . (1989). Proc. Natl. Acad. Sci. USA, 86, 7456–7459.
Weissman I, Papaioannou V and Gardner D . (1978). Differentiation of Normal and Neoplastic Hematopoietic Cells. Clarkson B, Marks PA and Till JE (eds). Cold Spring Harbor Laboratory: Cold Spring Harbor, NY, pp 33–47.
Xu M, Tsuji K and Ueda T . (1998). Blood, 92, 2032–2040.
Yoder MC, Hiatt K, Dutt P, Mukherjee P, Bodine DM and Orlic D . (1997). Immunity, 7, 335–344.
Yoder M and Hiatt K . (1997). Blood, 89, 2176–2183.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Yoder, M. Generation of HSCs in the embryo and assays to detect them. Oncogene 23, 7161–7163 (2004). https://doi.org/10.1038/sj.onc.1207931
Published:
Issue Date:
DOI: https://doi.org/10.1038/sj.onc.1207931
Keywords
This article is cited by
-
Developmental changes in hematopoietic stem cell properties
Experimental & Molecular Medicine (2013)
-
Expression of T‐cell receptor genes during early T‐cell development
Immunology & Cell Biology (2008)