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In vitro and in vivo expansion of hematopoietic stem cells

Oncogene volume 23, pages 7223–7232 (2004)Cite this article

Abstract

The capacity for sustained self-renewal – the generation of daughter cells having the same regenerative properties as the parent cell – is the defining feature of hematopoietic stem cells (HSCs). Strong evidence exists that self-renewal of HSC is under extrinsic biological control in vivo. A variety of cytokines, morphogenic ligands and associated signaling components influence self-renewal in culture and in vivo. Specific homeobox transcription factors act as powerful intrinsic agonists of HSC self-renewal in vitro and in vivo when supplied either as transduced cDNAs or as externally delivered proteins. These findings provide tools for deepening our knowledge of mechanism and for achievement of clinically useful levels of HSC expansion.

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Authors and Affiliations

  1. Laboratory of Molecular Genetics of Hemopoietic Stem Cells, Institute of Research in Immunology and Cancer, University of Montreal, CP 6128, Downtown Station, Montreal, H3C 3J7, Quebec, Canada

    Guy Sauvageau

  2. Department of Medicine, University of Montreal, Canada

    Guy Sauvageau

  3. Division of Hematology, Hospital Maisonneuve-Rosemont, Canada

    Guy Sauvageau

  4. Ontario Cancer Institute and the Department of Medical Biophysics, University of Toronto, Ontario, Canada

    Norman N Iscove

  5. Terry Fox Laboratory, British Columbia Cancer Agency, Vancouver, V5Z 1L3, British Columbia, Canada

    R Keith Humphries

  6. Department of Medicine, University of British Columbia, Vancouver, V5Z 4E3, British Columbia, Canada

    R Keith Humphries

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  1. Guy Sauvageau
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  2. Norman N Iscove
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  3. R Keith Humphries
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Correspondence to Guy Sauvageau or R Keith Humphries.

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Sauvageau, G., Iscove, N. & Humphries, R. In vitro and in vivo expansion of hematopoietic stem cells. Oncogene 23, 7223–7232 (2004). https://doi.org/10.1038/sj.onc.1207942

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