Oncogene

FIGURE 6

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E- and N-cadherin differ with respect to their associated p120ctn isoforms and their ability to suppress invasive growth in pancreatic cancer cells

Bjoern Seidel, Simone Braeg, Guido Adler, Doris Wedlich and Andre Menke

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Figure 6.

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Cadherin-associated p120ctn isoforms and their tyrosine phosphorylation. (a) Immunostaining of transfected MIA PaCa-2 cells with anti-p120ctn antibody demonstrated its localization at the cell membrane. Bar, 30 mum. (b) The overall concentration of p120ctn and of the different isoforms was not changed in the analysed cell clones. In addition, the phosphorylation of p120ctn isoforms was not significantly altered. (c) Isoforms and phosphorylation state of p120ctn associated with transfected cadherins was analysed by Western blotting after immunoprecipitation of E- or N-cadherin. The phosphorylation status of p120ctn was estimated by incubation with a phosphotyrosine-specific antibody. To correlate the resulting bands with p120ctn, the blots were reprobed with an anti-p120ctn antibody. Representative assays out of three independent experiments are shown

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