Oncogene

FIGURE 4

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Low p21Waf1/Cip1 protein level sensitizes testicular germ cell tumor cells to Fas-mediated apoptosis

Diana C J Spierings, Elisabeth G E de Vries, Alja J Stel, Nelina te Rietstap, Edo Vellenga and Steven de Jong

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Figure 4.

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p21 is mainly localized in the cytoplasm upon irradiation. Tera cells were irradiated or treated with cisplatin. After 24 h, nuclear and cytoplasmic proteins were isolated as described under 'Materials and methods' and analysed by immunoblotting for expression of p53, p21 and Rb. Actin is shown as a loading control. A representative example of at least three independent experiments is shown

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