FIGURE 3
FROM:
Transfection of a dominant-negative mutant NF-kB inhibitor (IkBm) represses p53-dependent apoptosis in acute lymphoblastic leukemia cells: interaction of IkBm and p53
Muxiang Zhou, Lubing Gu, Ningxi Zhu, William G Woods and Harry W Findley
BACK TO ARTICLEFigure 3.
Expression and regulation of p53 and IkB in response to chemotherapeutic agents. (a) EU-1/neo and EU-1/IkBm cells were treated with 0.5 
g/ml Dox or 0.1 g/ml VCR for the indicated time, and assayed for expression of p53, IkB
and IkBm by Western blot. (b) EU-1/neo and EU-1/IkBm cells were treated with 0.5 g/ml Dox for 2 h and assayed for expression of p53, IkB and IkBm in the cytosol and the nucleus by Western blot. (c) Binding of p53 to IkB and IkBm in vivo as examined by coimmunoprecipitation (IP) assay. Cytosolic proteins were extracted from EU-1/neo and EU-1/IkBm cells treated with 0.5 g/ml Dox and 0.1 g/ml VCR for different time periods as indicated, and then precipitated with anti-IkB antibody. The immunoprecipitated protein was analysed by Western blotting using anti-p53 antibody
