FIGURE 2
FROM:
Transfection of a dominant-negative mutant NF-kB inhibitor (IkBm) represses p53-dependent apoptosis in acute lymphoblastic leukemia cells: interaction of IkBm and p53
Muxiang Zhou, Lubing Gu, Ningxi Zhu, William G Woods and Harry W Findley
BACK TO ARTICLEFigure 2.
Effect of IkBm transfection on cell growth and sensitivity to apoptosis induced by chemotherapeutic drugs. (a) EU-1 cells transfected with IkBm (EU-1/IkBm) and control cells (EU-1 parental and EU-1/neo) were cultured in 10 ml of RPMI 1640 medium supplemented with 10% FBS at an initial concentration of 104/ml. Cells were counted at different time points. Data for the total number of cells (mean
s.d. for triplicate cultures) are shown. (b, c), EU-1/IkBm and EU-1/neo cells were cultured with different concentrations of Dox and VCR for 48 h, and cell survival was determined by XTT assay. Cell survival was expressed as the percentage of control (i.e. cultures without drugs). Data represent means.d. detected in triplicate experiments. (d) Western blot analysis for activation of caspase 3 and cleavage of its substrate PARP in EU-1/neo and EU-1/IkBm cells following treatment with Dox (0.5 
g/ml) and VCR (0.1 g/ml) for different time periods as indicated. Anti-caspase 3 recognizes both the unprocessed proprotease and the cleaved subunits. Anti-PARP detected a fragment cleaved from the PARP holoenzyme
