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Rare mutations of the DMBT1 gene in human astrocytic gliomas

Oncogene volume 21, pages 5956–5959 (2002)Cite this article

Abstract

The Deleted in Malignant Brain Tumors 1 gene (DMBT1) has been proposed as a tumor suppressor gene candidate in human brain tumors, based on the observation of homozygous deletions affecting the DMBT1 region or part of the gene. In order to support this hypothesis, we performed a mutational analysis of the entire coding region of DMBT1, employing SSCP analysis and direct DNA sequencing in a series of 79 astrocytic gliomas. Five somatic mutations were detected. Two mutations, one of which resulted in an amino acid exchange, occurred in glioblastomas. One pilocytic astrocytoma carried two missense mutations and another pilocytic astrocytoma contained a somatic mutation, not affecting the presumed protein. In addition, 21 of the 27 single nucleotide polymorphisms identified in this study have not been recognized previously. The data indicate, that small mutations are not a frequent finding in gliomas.

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Acknowledgements

We wish to thank Mrs C Spingies for excellent technical assistance. This study was supported by the Dr Mildred Scheel Stiftung für Krebsforschung (10-1318-De 1; 10-1260-Po 2), the Deutsche Krebshilfe (70-2385-Wi 2) and the Wilhelm Sander Stiftung (99.018.1).

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Authors and Affiliations

  1. Institute for Neuropathology, Charité Humboldt University, Berlin, D-13353, Germany

    Wolf Mueller & Andreas von Deimling

  2. Department of Molecular Genome Analysis, Deutsches Krebsforschungszentrum, Heidelberg, D-69120, Germany

    Jan Mollenhauer & Annemarie Poustka

  3. Department of Neurosurgery, Charité Humboldt University, Berlin, D-13353, Germany

    Florian Stockhammer

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  1. Wolf Mueller
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  2. Jan Mollenhauer
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  4. Annemarie Poustka
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  5. Andreas von Deimling
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Correspondence to Andreas von Deimling.

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Mueller, W., Mollenhauer, J., Stockhammer, F. et al. Rare mutations of the DMBT1 gene in human astrocytic gliomas. Oncogene 21, 5956–5959 (2002). https://doi.org/10.1038/sj.onc.1205733

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