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| Original Paper |
| E2Fs up-regulate expression of genes involved in DNA replication, DNA repair and mitosis |
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| Shirley Polagera, Yael Kalmaa, Eli Berkovich and Doron Ginsberg |
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Department of Molecular Cell Biology, The Weizmann Institute of Science, Rehovot 76100, Israel
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Correspondence to: D Ginsberg, Department of Molecular Cell Biology, The Weizmann Institute of Science, Rehovot 76100, Israel. E-mail: doron.ginsberg@weizmann.ac.il |
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aThese authors contributed equally to this research |
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| Abstract |
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The E2F family of transcription factors plays a pivotal role in the regulation of cell proliferation in higher eukaryotes. We used DNA microarrays and cell lines containing either inducible E2F-1 or inducible E2F-3 to identify novel E2F target genes. Our data indicate that E2F up-regulates the expression of genes not previously described as E2F target genes. A number of these E2F-regulated genes are involved in DNA replication, DNA repair and mitosis. These results suggest that E2F affects cell cycle progression both at S phase and during mitosis. Furthermore, our findings indicate that E2F-dependent gene activation may contribute to the cellular response to DNA damage. Oncogene (2002) 21, 437-446 DOI: 10.1038/sj/onc/1205102 |
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| Keywords |
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E2F; cell cycle; mitosis; DNA replication; DNA repair; DNA microarrays |
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| Received 24 July 2001; revised 29 October 2001; accepted 29 October 2001 |
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| 17 January 2002, Volume 21, Number 3, Pages 437-446 |
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