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Insulin-like growth factor I stimulates motility in human neuroblastoma cells

Oncogene volume 20, pages 7542–7550 (2001)Cite this article

Abstract

Motility is an important process that contributes to cancer cell spread. Growth factors are key regulators of motility in many cell types. Insulin-like growth factor I (IGF-I) causes SH-SY5Y human neuroblastoma cells to undergo dynamic morphological changes, leading to the extension of lamellipodia. IGF-I stimulated lamellipodia extension requires signaling through both phosphatidylinositol 3-kinase (PI3-K) and MAP kinase pathways. IGF-I, over a period of hours, stimulates SH-SY5Y and SHEP neuroblastoma cells to become more motile. While SH-SY5Y and SHEP cells use different insulin receptor substrate (IRS) isoforms to transduce signals from the IGF-I receptor, IGF-I has the same relative effect on the motility of both cell lines. Blocking the PI3-K and MAP kinase pathways attenuates the ability of IGF-I to increase motility. Overexpression of PTEN also attenuates IGF-I mediated motility. These results delineate some of the proximal events in the signaling mechanism utilized by IGF-I to stimulate cell motility.

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Acknowledgements

The authors acknowledge Yutein Chung for technical assistance, Kathleen Ignatowski and Cynthia van Golen for preparation of the SHEP-PTEN transfectants, and Judith Boldt for secretarial assistance. This work was supported by NIH RO1 NS38849 (EL Feldman) and 5T32 GM07863 and 5T32 CA09676 (GE Meyer).

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Authors and Affiliations

  1. Neuroscience Program, University of Michigan, Ann Arbor, Michigan, USA

    Gary E Meyer & Eva L Feldman

  2. Medical Scientist Training Program, University of Michigan, Ann Arbor, Michigan, USA

    Gary E Meyer

  3. Department of Cell and Developmental Biology, University of Michigan, Ann Arbor, Michigan, USA

    Eric Shelden

  4. Department of Neurology, University of Michigan, Ann Arbor, Michigan, USA

    Bhumsoo Kim & Eva L Feldman

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  1. Gary E Meyer
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  2. Eric Shelden
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  4. Eva L Feldman
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Correspondence to Eva L Feldman.

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Meyer, G., Shelden, E., Kim, B. et al. Insulin-like growth factor I stimulates motility in human neuroblastoma cells. Oncogene 20, 7542–7550 (2001). https://doi.org/10.1038/sj.onc.1204927

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