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| 13 September 2001, Volume 20, Number 41, Pages 5846-5855 |
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| Original Paper |
| Nrh, a human homologue of Nr-13 associates with Bcl-Xs and is an inhibitor of apoptosis |
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| Abdel Aouacheria1,a, Estelle Arnaud1,a, Séverine Venet1, Philippe Lalle1, Manolo Gouy2, Dominique Rigal3 and Germain Gillet1 |
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1Institut de Biologie et Chimie des Protéines, UMR 5086 CNRS/Université Claude Bernard, 7 passage du Vercors, F69367 Lyon cedex 07, France
2Biométrie et Biologie Evolutive, UMR 5558 CNRS/Université Claude Bernard, 43 Boulevard du 11 novembre, F69622 Villeurbanne cedex, France
3Etablissement de Transfusion Sanguine, passage du Vercors, F 69007 Lyon, France
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Correspondence to: G Gillet, Institut de Biologie et Chimie des Protéines, UMR 5086 CNRS/Université Claude Bernard, 7 passage du Vercors, F69367 Lyon cedex 07, France. E-mail: g.gillet@ibcp.fr
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aA Aouacheria and E Arnaud contributed equally to this work |
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| Abstract |
| In search of human homologues of the anti-apoptotic protein Nr-13, we have characterized a human EST clone that potentially encodes a protein, which is the closest homologue of Nr-13 among the Bcl-2 family members, to date known, in humans. Phylogenetic analyses suggest Human nrh, Mouse diva/boo and Quail nr-13 to be orthologous genes. The nrh gene has the same overall organization as nr-13 and diva/boo with one single intron interrupting the ORF at the level of the Bcl-2-homology domain BH2. RT-PCR-based analysis of nrh expression indicated that this gene is preferentially expressed in the lungs, the liver and the kidneys. Interestingly, two in frame ATG codons can lead potentially to the synthesis of two products, one of them lacking 10 aminoacids at the N-terminal end. Sequence alignment with Nr-13 and Diva/Boo in addition to secondary structure prediction of the nrh transcript suggested that the shortest protein will be preferentially synthetized. Immunohistochemical analyses have revealed that Nrh is associated with mitochondria and the nuclear envelope. Moreover, Nrh preferentially associates with the apoptosis accelerator Bcl-Xs and behaves as an inhibitor of apoptosis both in yeast and vertebrate cells. Oncogene (2001) 20, 5846-5855. |
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| Keywords |
| apoptosis; molecular cloning; Bcl-2 family; phylogeny |
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| Received 17 April 2001; revised 11 June 2001; accepted 18 June 2001 |
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| 13 September 2001, Volume 20, Number 41, Pages 5846-5855 |
| Table of contents Previous Abstract Next Full text PDF |
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