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Site-specific and temporally-regulated retinoblastoma protein dephosphorylation by protein phosphatase type 1

Oncogene volume 20, pages 3776–3785 (2001)Cite this article

Abstract

pRb is dephosphorylated at mitotic exit by the type 1 serine/threonine protein phosphatases (PP1). Here we demonstrate for the first time that mitotic pRb dephosphorylation is a sequential, temporally-regulated event. We also provide evidence that the three mammalian isoforms of PP1, α, γ-1, and δ, differ in their respective preferences for site-specific pRb dephosphorylation and that the mitotic and G1 PP1-isoform counterparts exhibit differential activities towards mitotic pRb. Finally, the physiological relevance of the striking contrast between the patterns of Thr821 and Thr826 dephosphorylation, sites known to be important for disrupting binding of LXCXE-containing proteins to pRb, is addressed.

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Acknowledgements

This work was supported by Research Project Grant # 98-108 from the American Cancer Society, The Sally Edelman and Harry Gardner Cancer Research Foundation (awarded to JW Ludlow), and Cancer Center Core Grant CA11198.

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Authors and Affiliations

  1. Department of Biochemistry and Biophysics, University of Rochester School of Medicine and Dentistry, Rochester, 14642, New York, NY, USA

    Ethel Rubin & John W Ludlow

  2. Department of Cell and Molecular Biology, Institute of Cancer Research, UK

    Sibylle Mittnacht

  3. Department of Experimental Pathology, University of Pisa, Pisa, Italy

    Emma Villa-Moruzzi

  4. University of Rochester Cancer Center, Rochester, 14642, New York, NY, USA

    John W Ludlow

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  1. Ethel Rubin
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  2. Sibylle Mittnacht
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  3. Emma Villa-Moruzzi
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Correspondence to John W Ludlow.

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Rubin, E., Mittnacht, S., Villa-Moruzzi, E. et al. Site-specific and temporally-regulated retinoblastoma protein dephosphorylation by protein phosphatase type 1. Oncogene 20, 3776–3785 (2001). https://doi.org/10.1038/sj.onc.1204518

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