 |
| Original Paper |
| DNA damage-induced phosphorylation of p53 at serine 20 correlates with p21 and Mdm-2 induction in vivo |
|
| James R Jabbur1,2, Peng Huang3 and Wei Zhang1,2 |
|
1Department of Pathology, The University of Texas MD Anderson Cancer Center, Houston, Texas, TX 77030, USA
2Department of Cancer Biology, The University of Texas MD Anderson Cancer Center, Houston, Texas, TX 77030, USA
3Department of Clinical Investigation, The University of Texas MD Anderson Cancer Center, Houston, Texas, TX 77030, USA
|
|
Correspondence to: W Zhang, Department of Pathology, Box 85, The University of Texas M.D. Anderson Cancer Center, 1515 Holcombe Blvd., Houston, Texas 77030, USA
|
|
| Abstract |
| We investigated the induction and physiological role of Ser20 phosphorylation of p53 in response to DNA damage caused by ionizing radiation (IR) or ultraviolet radiation (UV). A polyclonal antibody that specifically recognizes a p53 peptide containing phosphorylated Ser20 was generated and used to detect p53 phosphorylation at Ser20. Western blot analyses of p53 in four cell lines with this antibody revealed that the p53 protein was phosphorylated at Ser20 to a different extent after treatment with IR or UV. The phosphorylation of Ser20 of wild-type p53 correlated with enhanced induction of the p53 downstream target genes p21WAF1/Cip1 (p21) and mdm-2. These results suggest that DNA damage-induced phosphorylation of p53 at Ser20 enhances the transactivation function of p53 for p21 and mdm-2 in vivo. Oncogene (2000) 19, 6203-6208. |
|
| Keywords |
| phosphorylation; ionizing radiation; p53; p21 |
|
|
|
|
| Received 27 April 2000; revised 12 October 2000; accepted 12 October 2000 |
|
| 14 December 2000, Volume 19, Number 54, Pages 6203-6208 |
| Table of contents Previous Abstract Next Full text PDF |
|
