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Characterization of the human and mouse ETV1/ER81 transcription factor genes: role of the two alternatively spliced isoforms in the human

Oncogene volume 18, pages 6278–6286 (1999)Cite this article

Abstract

The Ets transcription factors of the PEA3 group – E1AF/PEA3, ETV1/ER81 and ERM – are almost identical in the ETS DNA-binding and the transcriptional acidic domains. To accelerate our understanding of the molecular basis of putative diseases linked to ETV1 such as Ewing's sarcoma we characterized the human ETV1 and the mouse ER81 genes. We showed that these genes are both encoded by 13 exons in more than 90 kbp genomic DNA, and that the classical acceptor and donor splicing sites are present in each junction except for the 5′ donor site of intron 9 where GT is replaced by TT. The genomic organization of the ETS and acidic domains in the human ETV1 and mouse ER81 (localized to chromosome 12) genes is similar to that observed in human ERM and human E1AF/PEA3 genes. Moreover, as in human ERM and human E1AF/PEA3 genes, a first untranslated exon is upstream from the first methionine, and the mouse ER81 gene transcription is regulated by a 1.8 kbp of genomic DNA upstream from this exon. In human, the alternative splicing of the ETV1 gene leads to the presence (ETV1α) or the absence (ETV1β) of exon 5 encoding the C-terminal part of the transcriptional acidic domain, but without affecting the alpha helix previously described as crucial for transactivation. We demonstrated here that the truncated isoform (human ETV1β) and the full-length isoform (human ETV1α) bind similarly specific DNA Ets binding sites. Moreover, they both activate transcription similarly through the PKA-transduction pathway, so suggesting that this alternative splicing is not crucial for the function of this protein as a transcription factor. The comparison of human ETV1α and human ETV1β expression in the same tissues, such as the adrenal gland or the bladder, showed no clear-cut differences. Altogether, these data open a new avenue of investigation leading to a better understanding of the functional role of this transcription factor.

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Acknowledgements

This work has been carried out on the basis of grants awarded by the `Centre National de la Recherche Scientifique' (France), the `Institut Pasteur de Lille', the `Association pour la Recherche contre le Cancer' (France) and by the `Fonds National de la Recherche Scientifique' (Belgium).

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Authors and Affiliations

  1. UMR 8526 CNRS, Institut Pasteur de Lille, Institut de Biologie de Lille, BP 447, 1 rue Calmette, Lille, 59021, Cedex, France

    Laurent Coutte, Didier Monté, Laurent Pouilly, Frédérique Dewitte, Jean-Luc Baert & Yvan de Launoit

  2. GSF, Forschungszentrum für Umwelt und Gesundheit GmbH, Institut für Säugertiergenetik, Neuherberg, Neuherberg, 1129, D-85758, Postfach, Germany

    Kenji Imai & Jerzy Adamski

  3. Laboratoire de Génétique Moléculaire, Faculté des Sciences Pharmaceutiques et Biologiques de Paris, Université René Descartes, 75006, Paris, France

    Michel Vidaud

  4. Laboratoire de Virologie Moléculaire, Faculté de Médecine, Université Libre de Bruxelles, CP 614, 808 route de Lennik, 1070, Brussels, Belgium

    Yvan de Launoit

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  1. Laurent Coutte
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Coutte, L., Monté, D., Imai, K. et al. Characterization of the human and mouse ETV1/ER81 transcription factor genes: role of the two alternatively spliced isoforms in the human. Oncogene 18, 6278–6286 (1999). https://doi.org/10.1038/sj.onc.1203020

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