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Identification of a gene at 16q24.3 that restores cellular senescence in immortal mammary tumor cells

Oncogene volume 18, pages 5100–5107 (1999)Cite this article

Abstract

We have mapped a cellular senescence gene, SEN16, within a genetic distance of 3 – 7 cM, at 16q24.3. Microcell mediated transfer of a normal human chromosome 16, 16q22-qter or 16q23-qter restored cellular senescence in four immortal cell lines, derived from human and rat mammary tumors. The resumption of indefinite cell proliferation, concordant with the segregation of the donor chromosome, confirmed the presence of a senescence gene at 16q23-qter. While microcell hybrids were maintained in selection medium to retain the donor chromosome, sporadic immortal revertant clones arose among senescent cells. Reversion to immortal growth could occur due to inactivation of the senescence gene either by a mutation or a deletion. The analysis for chromosome 16 specific DNA markers, in revertant clones of senescent microcell hybrids, revealed a consensus deletion, spanning a genetic interval of approximately 3 – 7 cM at 16q24.3.

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Acknowledgements

This work was supported by grants from the American Cancer Society (ACS#RPG-97-138-01-MGO) and the American Federation for Aging Research (A96130) to GP Kaur. DE Reddy was supported by a predoctoral fellowship from US Army Breast Cancer Research Program (DAMD# 17-96-1-6059).

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  1. Fels Institute for Cancer Research, 3307 North Broad Street, AHB Room 201, Temple University School of Medicine, Philadelphia, PA 19140, Pennsylvania, USA

    Deepthi E Reddy, Arbansjit K Sandhu, Jon K DeRiel, Raghbir S Athwal & Gursurinder P Kaur

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  1. Deepthi E Reddy
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Reddy, D., Sandhu, A., DeRiel, J. et al. Identification of a gene at 16q24.3 that restores cellular senescence in immortal mammary tumor cells. Oncogene 18, 5100–5107 (1999). https://doi.org/10.1038/sj.onc.1202888

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