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| Article |
| JNK activation is not required for Fas-mediated apoptosis |
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| Walter Low1, Anna Smith2, Alan Ashworth2 and Mary Collins1 |
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1Department of Immunology, University College London, Windeyer Building, 46 Cleveland Street, London W1P 6DB, UK
2Section of Gene Function and Regulation, Chester Beatty Laboratories, Institute of Cancer Research, 237 Fulham Road, London SW3 6JB, UK
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Correspondence to: Mary Collins, Department of Immunology, University College London, Windeyer Building, 46 Cleveland Street, London W1P 6DB, UK
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| Abstract |
| Fas ligation in the presence of cycloheximide induced Jun N-terminal kinase 1 (JNK1) and JNK2 phosphorylation, caspase activation and cell death in the IL-3-dependent cell line BAF3. Fas-mediated apoptosis was prevented by expression of dominant negative FADD but not inhibited by IL-3. To investigate the role of JNK activation in this process, we examined cells over-expressing a JNK-specific phosphatase M3/6. M3/6 prevented Fas stimulation of JNK, but did not affect Fas-mediated caspase activation or cell death, demonstrating that JNK activation is not required for these processes. |
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| Keywords |
| caspase; CD95; FADD; M3/6; IL-3 |
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| Received 2 October 1998; revised 11 January 1999; accepted 19 January 1999 |
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| 24 June 1999, Volume 18, Number 25, Pages 3737-3741 |
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