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| 26 February 1998, Volume 16, Number 8, Pages 1031-1040 |
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| Article |
| Identification of a BRCA1-associated kinase with potential biological relevance |
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| Teresa F Burke1, Kimberley S Cocke1, Stephanie J Lemke1, Ed Angleton2, Gerald W Becker2 and Richard P Beckmann1,a |
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1Cancer Research, Lilly Research Laboratories, Eli Lilly and Company, Lilly Corporate Center, Indianapolis, Indiana 46285, USA
2Biotechnology Divisions, Lilly Research Laboratories, Eli Lilly and Company, Lilly Corporate Center, Indianapolis, Indiana 46285, USA
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aAuthor for correspondence: |
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| Abstract |
| A biochemical approach was used to identify proteins which interact with human BRCA1. Through this work, a kinase activity which co-purifies with BRCA1 has been identified. This kinase activity, which phosphorylates BRCA1 in vitro, was originally identified in Sf9 insect cells but is also present in cells of human origin including breast and ovarian carcinoma cell lines. The BRCA1 kinase activity in vitro is associated with a fragment of BRCA1 encompassing amino acids 329-435. This peptide is also phosphorylated in various human cell lines. A computer-assisted sequence analysis revealed that this peptide was a potential substrate for phosphorylation by PKA, PKC, or CKII. However, phosphorylation by these kinases could not be demonstrated in vitro indicating the presence of another kinase activity. Phosphorylation in vitro requires a minimal domain of BRCA1 encompassing amino acids 379-408. Notably, deletion of this minimal domain abolishes growth suppression by BRCA1 indicating that this domain, as well as phosphorylation within this domain, may be important for BRCA1 function. |
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| Keywords |
| breast cancer; BRCA1; phosphorylation |
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| Received 14 May 1997; revised 3 October 1997; accepted 6 October 1997 |
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| 26 February 1998, Volume 16, Number 8, Pages 1031-1040 |
| Table of contents Previous Abstract Next Article PDF |
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