Proliferation of chicken neuroretina cells induced by v-src, in vitro, depends on activation of the E2F transcription factor

Abstract

Quiescent chicken or quail retina neuroblasts (NR) can be induced to proliferate actively, in culture, by the v-src oncoprotein. The chE2F-1 transcription factor, a physiological partner of the retinoblastoma gene product, is highly expressed in vivo, in dividing chicken neuroretina cells (CNR). It is sharply down-regulated as cells enter the post-mitotic differentiation stage, thus suggesting that E2F activity is a prerequisite for NR cell proliferation. In the present paper, transient expression assays of different forms of chE2F-1 were used to investigate the function of E2F for switching CNR cells from a quiescent to a proliferative state in vitro. Attempts to substitute the effects of v-src by an ectopic expression of E2F-1 were unsuccessful. However, in the same conditions, E2F-1 supports full growth of CEF in serum-depleted medium. Deletion mutants of E2F-1, with potential dominant-negative properties, were transfected in RSV infected CNR cells. One of these truncated mutants induces a G1 phase block in RSV-transformed CNR cells indicating that, although E2F-1 overexpression cannot overcome the cell proliferation block of post-mitotic CNR cells E2F-1, activity is an important component of the growth signal pathway delivered by v-src in these nervous cells.