FIGURES AND TABLES
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Acyl Coenzyme A Synthetase Regulation: Putative Role in Long-Chain Acyl Coenzyme A Partitioning
Yan-Lin Wang, Wen Guo, Yan Zang, Gordon C. Yaney, Gino Vallega, Lisa Getty-Kaushik, Paul Pilch, Konstantin Kandror and Barbara E. Corkey
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Figure 1.
Comparison of total ACSL activity in homogenates from isolated rat adipocytes. Adipocytes were isolated from young (60 grams) or adult (250 grams) male Sprague Dawley rats that were fed ad libitum, fasted 16 hours, or made diabetic by treatment with STZ. ACSL activity was measured in the homogenate by a radioisotopic assay measuring labeled palmitate incorporation into palmitoyl CoA. Values represent the mean 
SE of four to six separate animals. (*) p < 0.05.
Figure 2.
Effect of fasting on subcellular distribution of total ACSL activity in adipocyte fractions from adult fed and fasted rats. ACSL activity was determined in adipocytes separated into subcellular fractions using a sucrose gradient as described in "Research Methods and Procedures." Results are corrected for the total volume of each fraction. Values represent the mean SE of four to six separate animals. (*) p < 0.05.
Figure 3.
Effect of fasting on the specific activity of ACSL in subcellular fractions from adult fed and fasted rats. ACSL activity was determined in adipocytes separated into subcellular fractions using a sucrose gradient as described in "Research Methods and Procedures." Values represent the mean SE of four to six separate animals. (*) p < 0.05.
Figure 4.
Effect of insulin on the specific activity of ACSL in subcellular fractions from adult fed rats. ACSL activity was determined in adipocytes separated into subcellular fractions using a sucrose gradient as described in "Research Methods and Procedures." Values represent the mean SE of four to six separate animals. (*) p < 0.05.
Figure 5.
Western blot illustrating the effect of insulin on ACSL and Glut4 distribution in LDM and PM fractions from adult fed rats. Adipocyte membrane fractions (5 to 15 
g protein) were subjected to SDS-PAGE (9% gel) and Western blotting as described in "Research Methods and Materials." A similar distribution was found in four independent experiments.
Figure 6.
Comparison of oxidation and lipid synthesis in isolated rat adipocytes from fed, fasted, insulin-treated, and diabetic rats. Intact isolated adipocytes were from adult rats that were fed ad libitum, treated with insulin for 15 minutes, fasted 16 hours, or made diabetic by treatment with STZ. FFA conversion to CO2 and lipids was measured using radiolabeled palmitate. Values represent the mean SE of four to six separate animals. (*) p < 0.05.
