Research

This list contains the 50 most recently published research articles, including advance online publication articles that have not yet been published in a journal issue.

Showing: 1–25 of 50

AOP
  1. Chromatin-enriched lncRNAs can act as cell-type specific activators of proximal gene transcription AOP

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    Functional characterization of chromatin enriched lncRNAs (cheRNAs) reveals their role as cis-acting transcriptional activators that couple enhancers at sites of cheRNA synthesis to promoters of proximal target genes.

  2. Structural basis for the cooperative allosteric activation of the free fatty acid receptor GPR40 AOP

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    Crystal structures of hGPR40, a target for treatment of type 2 diabetes, bound to a partial and an allosteric agonist explain the binding cooperativity between these ligands and present new opportunities for structure-guided drug design.

  3. Structural analysis of MDM2 RING separates degradation from regulation of p53 transcription activity AOP

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    MDM2 mutations that prevent E2–ubiquitin binding without altering RING domain structure lead to loss of E3-ligase activity, while the ability to limit p53 transcriptional activity is retained, allowing cells to respond more quickly to cellular stress.

  4. 5-Formylcytosine does not change the global structure of DNA

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    X-ray crystallography and NMR analysis demonstrate that, contrary to previous observations, fC does not significantly alter DNA structure, thus suggesting an alternative basis for recognition of fC-DNA by epigenome-modifying enzymes.

  5. The myosin mesa and the basis of hypercontractility caused by hypertrophic cardiomyopathy mutations

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    A working model for β-cardiac myosin in the sequestered state and binding assays reveal interactions between the myosin head and tail that are disrupted by mutations associated with hypertrophic cardiomyopathy.

  6. Human CTP synthase filament structure reveals the active enzyme conformation

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    The human enzyme CTP synthase forms polymeric filaments with increased catalytic activity, in contrast to the inactive filaments formed by bacterial CTP synthase. Cryo-EM and crystallographic analyses explain the structural bases for those different behaviors.

  7. ADAR1 controls apoptosis of stressed cells by inhibiting Staufen1-mediated mRNA decay

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    In stressed cells, the ADAR1p110 isoform is phosphorylated and translocated from the nucleus to the cytoplasm, where it protects transcripts with 3′-UTR dsRNA structures from Staufen1-mediated decay, thus suppressing cellular apoptosis.

    See also: News and Views by Reyad A Elbarbary et al.

  8. Active and poised promoter states drive folding of the extended HoxB locus in mouse embryonic stem cells

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    Homotypic interactions between active and Polycomb-repressed promoters co-occurring in the same DNA fiber, rather than CTCF occupancy, explain the 3D HoxB folding pattern.

    See also: News and Views by Swastika Sanyal et al.

  9. Parkin–phosphoubiquitin complex reveals cryptic ubiquitin-binding site required for RBR ligase activity

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    The human RBR E3 ligase Parkin is captured in complex with phosphoubiquitin, revealing a cryptic ubiquitin-binding site and indicating a mechanism of cooperation between RBR modules for activation.

  10. Structural basis for lipopolysaccharide extraction by ABC transporter LptB2FG

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    The crystal structure of LptB2FG, an ABC transporter that extracts LPS from the bacterial inner membrane and transports it to the outer membrane, indicates a transport mechanism distinct from classical ABC transporters.

  11. Structure of the 40S–ABCE1 post-splitting complex in ribosome recycling and translation initiation

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    Cryo-EM structures of the yeast 40S in complex with ribosome-splitting protein ABCE1, along with functional analyses, reveal that the FeS cluster domain undergoes a 150° rotation to dissociate ribosomal subunits.

  12. A cytosolic Ezh1 isoform modulates a PRC2–Ezh1 epigenetic adaptive response in postmitotic cells

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    A cytosolic isoform of Ezh1 that lacks the catalytic domain controls nuclear PRC2 activity in response to atrophic oxidative stress in skeletal muscle cells by trapping Eed in the cytoplasm.

    See also: News and Views by Marjorie Brand et al.

  13. Genome-wide mapping of long-range contacts unveils clustering of DNA double-strand breaks at damaged active genes

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    Capture Hi-C analysis reveals that DNA double-strand breaks within transcriptionally active regions of the human genome form clusters that exhibit delayed repair in the G1 phase of the cell cycle.