Abstract
The mRNA codon in the ribosomal A-site is recognized by aminoacyl-tRNA (aa-tRNA) in a ternary complex with elongation factor Tu (EF-Tu) and GTP. Here we report the 13 Å resolution three-dimensional reconstruction determined by cryo-electron microscopy of the kirromycin-stalled codon-recognition complex. The structure of the ternary complex is distorted by binding of the tRNA anticodon arm in the decoding center. The aa-tRNA interacts with 16S rRNA, helix 69 of 23S rRNA and proteins S12 and L11, while the sarcin-ricin loop of 23S rRNA contacts domain 1 of EF-Tu near the nucleotide-binding pocket. These results provide a detailed snapshot view of an important functional state of the ribosome and suggest mechanisms of decoding and GTPase activation.
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Acknowledgements
We thank C. Schillings, A. Böhm, S. Möbitz and P. Striebeck for expert technical assistance and M. Schatz and R. Schmidt for IMAGIC-5 (ref. 45) support. Work in our laboratories is supported by the Deutsche Forschungsgemeinschaft, the Alfried Krupp von Bohlen und Halbach-Stiftung, the Higher Education Funding Council for England and the Biotechnology and Biological Sciences Research Council.
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Stark, H., Rodnina, M., Wieden, HJ. et al. Ribosome interactions of aminoacyl-tRNA and elongation factor Tu in the codon-recognition complex. Nat Struct Mol Biol 9, 849–854 (2002). https://doi.org/10.1038/nsb859
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DOI: https://doi.org/10.1038/nsb859
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