 | Figure 4
Nature Structural Biology
8, 312 - 316 (2001)
doi:10.1038/86181
The homing endonuclease I-CreI uses three metals, one of which is shared between the two active sitesBrett S. Chevalier, Raymond J. Monnat Jr.
& Barry L. Stoddard | | | | Figure 4. Schematic of the active site interactions and solvent network.
a, The substrate complex. b, The cleaved product complex. The residue numbers and labels are the same as in Figs 1
−3. Water molecules are numbered consistently with the corresponding PDB files and are shown in blue with the exception of the proposed nucleophilie water molecule (orange 24) in the top panel. Bond distances are given in Å. All direct contacts between the bound metal ions and protein side chains and water molecules and the scissile phosphate are shown by thin green bonds; the contacts to phosphate atoms on the opposite DNA strand are omitted for clarity. The metal ions display octahedral coordination, with ligand bond distances ranging from 2.0 to 2.1 Å for magnesium and 2.4 to 2.5 Å for calcium.
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