Restriction endonucleases: one of these things is not like the others
Eric A. Galburt
& Barry L. Stoddard
Eric A. Galburt and Barry L. Stoddard are in the Program
in Biomolecular Structure and Design, University of Washington and the
Fred Hutchinson Cancer Research Center, 1100 Fairview Ave. N.,
Seattle, Washington 98109, USA.
Correspondence should be addressed to Barry L. Stoddard bstoddar@fhcrc.org
The crystal structure of the restriction endonuclease BglII in complex
with its DNA target site has been determined. The DNA binding mode and chemistry
of catalysis are observed to differ from BamHI which cleaves a similar
target site. These observations indicate that more divergence has occurred
within this family of proteins than originally thought.
