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Look Ma, no PCNA: how DNA polymerase ε synthesizes long stretches of DNA without a processivity factor

Nature Structural & Molecular Biology volume 21, pages 12–14 (2014)Cite this article

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The long-awaited crystal structure of Saccharomyces cerevisiae DNA polymerase ε reveals a unique domain never before observed in B-family DNA polymerases. This novel domain endows polymerase ε with a capacity for highly processive DNA synthesis.

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Figure 1: Comparison of polymerase ε and polymerase δ structural domains and bound DNA.
Figure 2: Close-up view of the P domain and metal-binding region.

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Acknowledgements

Work in the authors' laboratory was supported by US National Institutes of Health grant R01 CA052040.

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  1. Sylvie Doublié and Karl E. Zahn are in the Department of Microbiology and Molecular Genetics, University of Vermont, Burlington, Vermont, USA.,

    Karl E Zahn & Sylvie Doublié

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  1. Karl E Zahn
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  2. Sylvie Doublié
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Correspondence to Sylvie Doublié.

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The authors declare no competing financial interests.

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Zahn, K., Doublié, S. Look Ma, no PCNA: how DNA polymerase ε synthesizes long stretches of DNA without a processivity factor. Nat Struct Mol Biol 21, 12–14 (2014). https://doi.org/10.1038/nsmb.2749

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