The plasma membrane delimits the cell and controls material and information exchange between itself and the environment. How different plasma-membrane processes are coordinated and how the relative abundance of plasma-membrane lipids and proteins is homeostatically maintained are not yet understood. Here, we used a quantitative genetic interaction map, or E-MAP, to functionally interrogate a set of ~400 genes involved in various aspects of plasma-membrane biology, including endocytosis, signaling, lipid metabolism and eisosome function. From this E-MAP, we derived a set of 57,799 individual interactions between genes functioning in these various processes. Using triplet genetic motif analysis, we identified a new component of the eisosome, Eis1, and linked the poorly characterized gene EMP70 to endocytic and eisosome function. Finally, we implicated Rom2, a GDP/GTP exchange factor for Rho1 and Rho2, in the regulation of sphingolipid metabolism.
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- Supplementary Text and Figures (10M)
Supplementary Figures 1–5, Supplementary Tables 3–7
- Supplementary Table 1 (68K)
Classification of genes used for Figure 1a
- Supplementary Table 2 (13M)
Correlations of genes pairs in the plasma membrane E-MAP used in Figures 1b and 1c.
- Supplementary Video 1 (12M)
Localization of Emp70-GFP.
- Supplementary Video 2 (12M)
Localization of Emp70-GFP in respect to Lsp1-RFPmars.