Figure 3 - mRNA uridylation is Cid1-dependent, and impairment of decapping increases uridylation on decapped messages.
From the following article
Decapping is preceded by 3' uridylation in a novel pathway of bulk mRNA turnover
Olivia S Rissland & Chris J Norbury
Nature Structural & Molecular Biology 16, 616 - 623 (2009) Published online: 10 May 2009
doi:10.1038/nsmb.1601
(a) The percentage of adenylated, decapped act1 sequences that contain (black) or lack (white) terminal uridyl residues is plotted for sequences isolated from wild-type (WT), cid1
and dcp1-ts cells (n = 40, 29 and 20, respectively). (b,c) Poly(A) tail lengths, binned into groups of 10 nt, of decapped (b) and capped (c) act1 sequences isolated from wild-type (black; n = 40 and 20, respectively) and cid1 (white; n = 29 and 36, respectively) cells are compared. (d,e) Poly(A) tail lengths, binned into groups of 10 nt, of decapped (d) and capped (e) act1 sequences isolated from wild-type (black; n = 40 and 20, respectively) and dcp1-ts (white; n = 29 and 18, respectively) cells are compared.
