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Nature Structural & Molecular Biology 16, 1123 - 1124 (2009)
doi:10.1038/nsmb1109-1123
Tracking rates of transcription and splicing in vivo
M Behfar Ardehali1 & John T Lis1
- M. Behfar Ardehali and John T. Lis are in the Department of Molecular Biology and Genetics, Cornell University, Ithaca, New York, USA.
Correspondence to: John T Lis1 e-mail: jtl10@cornell.edu
Abstract
A relatively simple but powerful method to measure RNA polymerase II transcription elongation as well as co-transcriptional RNA splicing rates at many genes in vivo is described in this issue. The results demonstrate a rather uniform, and high, elongation rate at large human genes and co-transcriptional pre-mRNA splicing of both U2- and U12-dependent primary transcripts.
