Article abstract


Nature Structural & Molecular Biology 15, 714 - 721 (2008)
Published online: 15 June 2008 | Corrected online: 22 June 2008 | doi:10.1038/nsmb.1440

Capped small RNAs and MOV10 in human hepatitis delta virus replication

Dirk Haussecker1, Dan Cao1, Yong Huang1, Poornima Parameswaran1, Andrew Z Fire1 & Mark A Kay1


The evolutionary origin of human hepatitis delta virus (HDV) replication by RNA-directed transcription is unclear. Here we identify two species of 5'-capped, approx18–25-nucleotide small RNAs. One was of antigenomic polarity, corresponding to the 5' end of hepatitis delta antigen (HDAg) mRNA, and interacted with HDAg and RNA polymerase II (Pol II), whereas the other mapped to a structurally analogous region on the genomic RNA hairpin. An HDAg-interaction screen indicated that HDAg interacts with MOV10, the human homolog of the Arabidopsis thaliana RNA amplification factor gene SDE3 and Drosophila melanogaster RISC-maturation factor gene Armitage (armi). MOV10 knockdown inhibited HDV replication, but not HDAg mRNA translation, further supporting a role for MOV10 in RNA-directed transcription. Together, our studies define RNA hairpins as critical elements for the initiation of HDV-related, RNA-directed transcription. The identification of capped small RNAs and the involvement of MOV10 in HDV replication further suggest a conserved mechanism related to RNA-directed transcription in lower eukaryotes.

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  1. Departments of Pediatrics and Genetics, Stanford University, 300 Pasteur Dr., Rm. G305, Stanford, California 94305, USA.

Correspondence to: Mark A Kay1 e-mail: markay@stanford.edu

* In the version of this article initially published online, the top panel in Figure 3d was mistakenly replaced with a panel from Figure 3c. The error has been corrected for all versions of this article.

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