Article abstract
Nature Structural & Molecular Biology 15, 477 - 484 (2008)
Published online: 13 April 2008 | doi:10.1038/nsmb.1419
ATP-dependent chromatin remodeling shapes the DNA replication landscape
Jack A Vincent1, Tracey J Kwong1 & Toshio Tsukiyama1
Abstract
The eukaryotic DNA replication machinery must traverse every nucleosome in the genome during S phase. As nucleosomes are generally inhibitory to DNA-dependent processes, chromatin structure must undergo extensive reorganization to facilitate DNA synthesis. However, the identity of chromatin-remodeling factors involved in replication and how they affect DNA synthesis is largely unknown. Here we show that two highly conserved ATP-dependent chromatin-remodeling complexes in Saccharomyces cerevisiae, Isw2 and Ino80, function in parallel to promote replication fork progression. As a result, Isw2 and Ino80 have especially important roles for replication of late-replicating regions during periods of replication stress. Both Isw2 and Ino80 complexes are enriched at sites of replication, suggesting that these complexes act directly to promote fork progression. These findings identify ATP-dependent chromatin-remodeling complexes that promote DNA replication and define a specific stage of replication that requires remodeling for normal function.
- Division of Basic Sciences, Fred Hutchinson Cancer Research Center, 1100 Fairview Avenue North, Seattle, Washington 98109, USA, and Molecular and Cellular Biology Program, University of Washington and Fred Hutchinson Cancer Research Center, Box 357275, Seattle, Washington 98195, USA.
Correspondence to: Toshio Tsukiyama1 e-mail: ttsukiya@fhcrc.org
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