Promoting creativity and innovation - p425

doi:10.1038/nsmb0508-425

If the United States is losing its competitive edge in science and technology, how can we begin to reverse that trend?

Full Text - Promoting creativity and innovation | PDF (203 KB) - Promoting creativity and innovation

Modifications target spliceosome dynamics - pp426 - 428

Corina Maeder & Christine Guthrie

doi:10.1038/nsmb0508-426

The splicing pathway is dominated by ATP-dependent RNA rearrangements promoted by DEAD-box helicases. Post-translational modifications have now been implicated in the regulation of two DEAD-box proteins that are required for catalytic activation of the spliceosome.

Full Text - Modifications target spliceosome dynamics | PDF (4,098 KB) - Modifications target spliceosome dynamics

See also: Article by Mathew et al. | Article by Bellare et al.

The type II secretion arrowhead: the structure of GspI–GspJ–GspK - pp428 - 430

Katrina T Forest

doi:10.1038/nsmb0508-428

A heterotrimeric complex of minor pseudopilins from the type II secretion system has been identified and its crystal structure solved. Although each subunit shares the same overall - fold as other characterized (pseudo)pilins, GspK has a unique large -helical domain inserted between two canonical -strands. The structure constrains models for pseudopilus assembly.

Full Text - The type II secretion arrowhead: the structure of GspI–GspJ–GspK | PDF (3,755 KB) - The type II secretion arrowhead: the structure of GspI–GspJ–GspK

See also: Article by Korotkov & Hol

DNA damage: a histone-code mediator leaves the stage - pp430 - 432

Jiri Lukas & Jiri Bartek

doi:10.1038/nsmb0508-430

Chromosomal breaks destabilize the genome and can cause developmental defects and diseases such as cancer. New work suggests that, shortly after DNA damage, dissociation of the histone binding protein HP1 from chromatin facilitates restoration of genome integrity.

Full Text - DNA damage: a histone-code mediator leaves the stage | PDF (397 KB) - DNA damage: a histone-code mediator leaves the stage

New clues to actin function in chromatin regulation - pp432 - 433

Aaron J Gottschalk, Ronald C Conaway & Joan Weliky Conaway

doi:10.1038/nsmb0508-432

Since the discovery that actin and actin-related proteins (ARPs) reside in the nucleus as integral subunits of chromatin-modifying and chromatin-remodeling complexes, efforts to uncover their roles in chromatin regulation have met with limited success. In a new study, the previously mysterious helicase-SANT–associated (HSA) domain found in many chromatin regulatory complexes is shown to act as a module that directs recruitment and contributes to the action of actin and ARPs in chromatin regulation.

Full Text - New clues to actin function in chromatin regulation | PDF (325 KB) - New clues to actin function in chromatin regulation

See also: Article by Szerlong et al.

Research highlights - p434

doi:10.1038/nsmb0508-434

Full Text - Research highlights | PDF (132 KB) - Research highlights

Phosphorylation of human PRP28 by SRPK2 is required for integration of the U4/U6-U5 tri-snRNP into the spliceosome - pp435 - 443

Rebecca Mathew, Klaus Hartmuth, Sina Möhlmann, Henning Urlaub, Ralf Ficner & Reinhard Lührmann

doi:10.1038/nsmb.1415

Abstract - | Full Text - Phosphorylation of human PRP28 by SRPK2 is required for integration of the U4/U6-U5 tri-snRNP into the spliceosome | PDF (877 KB) - Phosphorylation of human PRP28 by SRPK2 is required for integration of the U4/U6-U5 tri-snRNP into the spliceosome | Supplementary information

See also: News and Views by Maeder & Guthrie

A role for ubiquitin in the spliceosome assembly pathway - pp444 - 451

Priya Bellare, Eliza C Small, Xinhua Huang, James A Wohlschlegel, Jonathan P Staley & Erik J Sontheimer

doi:10.1038/nsmb.1401

Abstract - | Full Text - A role for ubiquitin in the spliceosome assembly pathway | PDF (835 KB) - A role for ubiquitin in the spliceosome assembly pathway | Supplementary information

See also: News and Views by Maeder & Guthrie

Membrane-dependent signal integration by the Ras activator Son of sevenless - pp452 - 461

Jodi Gureasko, William J Galush, Sean Boykevisch, Holger Sondermann, Dafna Bar-Sagi, Jay T Groves & John Kuriyan

doi:10.1038/nsmb.1418

Abstract - | Full Text - Membrane-dependent signal integration by the Ras activator Son of sevenless | PDF (1,352 KB) - Membrane-dependent signal integration by the Ras activator Son of sevenless | Supplementary information

Structure of the GspK–GspI–GspJ complex from the enterotoxigenic Escherichia coli type 2 secretion system - pp462 - 468

Konstantin V Korotkov & Wim G J Hol

doi:10.1038/nsmb.1426

• PDB code

  • 3CI0

• 3D view

  • 3CI0

Abstract - | Full Text - Structure of the GspK–GspI–GspJ complex from the enterotoxigenic Escherichia coli type 2 secretion system | PDF (1,412 KB) - Structure of the GspK–GspI–GspJ complex from the enterotoxigenic Escherichia coli type 2 secretion system | Supplementary information

See also: News and Views by Forest

The HSA domain binds nuclear actin-related proteins to regulate chromatin-remodeling ATPases - pp469 - 476

Heather Szerlong, Kaede Hinata, Ramya Viswanathan, Hediye Erdjument-Bromage, Paul Tempst & Bradley R Cairns

doi:10.1038/nsmb.1403

Abstract - | Full Text - The HSA domain binds nuclear actin-related proteins to regulate chromatin-remodeling ATPases | PDF (1,110 KB) - The HSA domain binds nuclear actin-related proteins to regulate chromatin-remodeling ATPases | Supplementary information

See also: News and Views by Gottschalk et al.

ATP-dependent chromatin remodeling shapes the DNA replication landscape - pp477 - 484

Jack A Vincent, Tracey J Kwong & Toshio Tsukiyama

doi:10.1038/nsmb.1419

Abstract - | Full Text - ATP-dependent chromatin remodeling shapes the DNA replication landscape | PDF (2,311 KB) - ATP-dependent chromatin remodeling shapes the DNA replication landscape | Supplementary information

Sequence-directed DNA export guides chromosome translocation during sporulation in Bacillus subtilis - pp485 - 493

Jerod L Ptacin, Marcelo Nollmann, Eric C Becker, Nicholas R Cozzarelli, Kit Pogliano & Carlos Bustamante

doi:10.1038/nsmb.1412

Abstract - | Full Text - Sequence-directed DNA export guides chromosome translocation during sporulation in Bacillus subtilis | PDF (1,052 KB) - Sequence-directed DNA export guides chromosome translocation during sporulation in Bacillus subtilis | Supplementary information

Signal sequence–independent membrane targeting of ribosomes containing short nascent peptides within the exit tunnel - pp494 - 499

Thomas Bornemann, Johannes Jöckel, Marina V Rodnina & Wolfgang Wintermeyer

doi:10.1038/nsmb.1402

Abstract - | Full Text - Signal sequence–independent membrane targeting of ribosomes containing short nascent peptides within the exit tunnel | PDF (652 KB) - Signal sequence–independent membrane targeting of ribosomes containing short nascent peptides within the exit tunnel

The structural basis for cap binding by influenza virus polymerase subunit PB2 - pp500 - 506

Delphine Guilligay, Franck Tarendeau, Patricia Resa-Infante, Rocío Coloma, Thibaut Crepin, Peter Sehr, Joe Lewis, Rob W H Ruigrok, Juan Ortin, Darren J Hart & Stephen Cusack

doi:10.1038/nsmb.1421

• PDB code

  • 2VQZ

• 3D view

  • 2VQZ

Abstract - | Full Text - The structural basis for cap binding by influenza virus polymerase subunit PB2 | PDF (870 KB) - The structural basis for cap binding by influenza virus polymerase subunit PB2 | Supplementary information

Aminoacylation of tRNA with phosphoserine for synthesis of cysteinyl-tRNA^Cys - pp507 - 514

Chun-Mei Zhang, Cuiping Liu, Simon Slater & Ya-Ming Hou

doi:10.1038/nsmb.1423

Abstract - | Full Text - Aminoacylation of tRNA with phosphoserine for synthesis of cysteinyl-tRNACys | PDF (957 KB) - Aminoacylation of tRNA with phosphoserine for synthesis of cysteinyl-tRNACys | Supplementary information

DNA apurinic-apyrimidinic site binding and excision by endonuclease IV - pp515 - 522

Elsa D Garcin, David J Hosfield, Sunil A Desai, Brian J Haas, Magnar Björas, Richard P Cunningham & John A Tainer

doi:10.1038/nsmb.1414

• PDB code

  • 2NQH
  • 2NQJ
  • 2NQ9

• 3D view

  • 2NQH
  • 2NQJ
  • 2NQ9

Abstract - | Full Text - DNA apurinic-apyrimidinic site binding and excision by endonuclease IV | PDF (1,471 KB) - DNA apurinic-apyrimidinic site binding and excision by endonuclease IV | Supplementary information

Mouse Eri1 interacts with the ribosome and catalyzes 5.8S rRNA processing - pp523 - 530

K Mark Ansel, William A Pastor, Nicola Rath, Ariya D Lapan, Elke Glasmacher, Christine Wolf, Laura C Smith, Nikoletta Papadopoulou, Edward D Lamperti, Mamta Tahiliani, Joachim W Ellwart, Yujiang Shi, Elisabeth Kremmer, Anjana Rao & Vigo Heissmeyer

doi:10.1038/nsmb.1417

Abstract - | Full Text - Mouse Eri1 interacts with the ribosome and catalyzes 5.8S rRNA processing | PDF (632 KB) - Mouse Eri1 interacts with the ribosome and catalyzes 5.8S rRNA processing | Supplementary information

See also: Brief Communication by Gabel & Ruvkun

The exonuclease ERI-1 has a conserved dual role in 5.8S rRNA processing and RNAi - pp531 - 533

Harrison W Gabel & Gary Ruvkun

doi:10.1038/nsmb.1411

Abstract - | Full Text - The exonuclease ERI-1 has a conserved dual role in 5.8S rRNA processing and RNAi | PDF (233 KB) - The exonuclease ERI-1 has a conserved dual role in 5.8S rRNA processing and RNAi | Supplementary information

See also: Article by Ansel et al.

A conserved rRNA methyltransferase regulates ribosome biogenesis - pp534 - 536

Zhili Xu, Heather C O'Farrell, Jason P Rife & Gloria M Culver

doi:10.1038/nsmb.1408

Abstract - | Full Text - A conserved rRNA methyltransferase regulates ribosome biogenesis | PDF (513 KB) - A conserved rRNA methyltransferase regulates ribosome biogenesis | Supplementary information