Table of contents
March 2008, Volume 15 No 3 pp217-330
About the coverEditorial
Shaping the discussion - p217
doi:10.1038/nsmb0308-217
The current move toward a presidential debate highlighting science and technology may at least provide the impetus for political discussion in these areas to go beyond hot button issues.
Full Text - Shaping the discussion | PDF (217 KB) - Shaping the discussion
News and Views
Forging a proteasome 
-ring with dedicated proteasome chaperones - pp218 - 220
Rina Rosenzweig & Michael H Glickman
doi:10.1038/nsmb0308-218
Assembly of the 34-subunit, 2.5 megadalton 26S proteasome starts with formation of the seven-membered 
-ring. A set of newly identified proteasome chaperones serves as a clamp to seal -rings with the correct composition. By regulating the efficiency and outcome of this crucial step in proteasome biogenesis, these dedicated proteasome chaperones apparently partake in the stress response and in adaptation to intracellular proteolysis needs.
Full Text - Forging a proteasome -ring with dedicated proteasome chaperones | PDF (2,051 KB) - Forging a proteasome -ring with dedicated proteasome chaperones
See also: Article by Yashiroda et al. | Article by Kusmierczyk et al.
Ankyrin for methylated lysines - pp221 - 222
Michael M Brent & Ronen Marmorstein
doi:10.1038/nsmb0308-221
The ankyrin repeats of the G9a and GLP histone methyltransferases have now been shown to be binding modules for mono- and dimethyllysine histone H3 lysine 9 (H3K9), revealing a new function for an ankyrin repeat domain and showing that a polypeptide chain can both create and recognize the same histone mark.
Full Text - Ankyrin for methylated lysines | PDF (267 KB) - Ankyrin for methylated lysines
See also: Article by Collins et al.
A purified catalytically competent spliceosome - pp222 - 224
Maria M Konarska
doi:10.1038/nsmb0308-222
The compositional complexity of the spliceosome creates a serious obstacle for its experimental analysis. Purification of a compositionally defined splicing complex C capable of completing the second step of splicing in the absence of additional proteins opens the door for future mechanistic and structural analyses.
Full Text - A purified catalytically competent spliceosome | PDF (196 KB) - A purified catalytically competent spliceosome
When a domain is not a domain - pp224 - 226
Sang Youn Park & Steven E Shoelson
doi:10.1038/nsmb0308-224
The kinase regulatory-loop binding (KRLB) region of insulin receptor substrate 2 was originally thought to be a new type of domain with binding properties similar to phosphotyrosine binding (PTB) domains. A crystallographic study now shows that KRLB is actually a short peptide segment that binds to the insulin receptor using an extended series of contacts that mimic both autoinhibitory loop and ATP binding to its catalytic cleft.
Full Text - When a domain is not a domain | PDF (336 KB) - When a domain is not a domain
See also: Article by Wu et al.
Research Highlights
Research Highlights - p227
doi:10.1038/nsmb0308-227
Full Text - Research Highlights | PDF (167 KB) - Research Highlights
Articles
Crystal structure of a chaperone complex that contributes to the assembly of yeast 20S proteasomes - pp228 - 236
Hideki Yashiroda, Tsunehiro Mizushima, Kenta Okamoto, Tomie Kameyama, Hidemi Hayashi, Toshihiko Kishimoto, Shin-ichiro Niwa, Masanori Kasahara, Eiji Kurimoto, Eri Sakata, Kenji Takagi, Atsuo Suzuki, Yuko Hirano, Shigeo Murata, Koichi Kato, Takashi Yamane & Keiji Tanaka
doi:10.1038/nsmb.1386
Abstract - | Full Text - Crystal structure of a chaperone complex that contributes to the assembly of yeast 20S proteasomes | PDF (984 KB) - Crystal structure of a chaperone complex that contributes to the assembly of yeast 20S proteasomes | Supplementary information
See also: News and Views by Rosenzweig & Glickman
A multimeric assembly factor controls the formation of alternative 20S proteasomes - pp237 - 244
Andrew R Kusmierczyk, Mary J Kunjappu, Minoru Funakoshi & Mark Hochstrasser
doi:10.1038/nsmb.1389
Abstract - | Full Text - A multimeric assembly factor controls the formation of alternative 20S proteasomes | PDF (586 KB) - A multimeric assembly factor controls the formation of alternative 20S proteasomes | Supplementary information
See also: News and Views by Rosenzweig & Glickman
The ankyrin repeats of G9a and GLP histone methyltransferases are mono- and dimethyllysine binding modules - pp245 - 250
Robert E Collins, Jeffrey P Northrop, John R Horton, David Y Lee, Xing Zhang, Michael R Stallcup & Xiaodong Cheng
doi:10.1038/nsmb.1384
Abstract - | Full Text - The ankyrin repeats of G9a and GLP histone methyltransferases are mono- and dimethyllysine binding modules | PDF (454 KB) - The ankyrin repeats of G9a and GLP histone methyltransferases are mono- and dimethyllysine binding modules | Supplementary information
See also: News and Views by Brent & Marmorstein
Structural and biochemical characterization of the KRLB region in insulin receptor substrate-2 - pp251 - 258
Jinhua Wu, Yolanda D Tseng, Chong-Feng Xu, Thomas A Neubert, Morris F White & Stevan R Hubbard
doi:10.1038/nsmb.1388
Abstract - | Full Text - Structural and biochemical characterization of the KRLB region in insulin receptor substrate-2 | PDF (607 KB) - Structural and biochemical characterization of the KRLB region in insulin receptor substrate-2 | Supplementary information
See also: News and Views by Park & Shoelson
MicroRNAs control de novo DNA methylation through regulation of transcriptional repressors in mouse embryonic stem cells - pp259 - 267
Lasse Sinkkonen, Tabea Hugenschmidt, Philipp Berninger, Dimos Gaidatzis, Fabio Mohn, Caroline G Artus-Revel, Mihaela Zavolan, Petr Svoboda & Witold Filipowicz
doi:10.1038/nsmb.1391
Abstract - | Full Text - MicroRNAs control de novo DNA methylation through regulation of transcriptional repressors in mouse embryonic stem cells | PDF (668 KB) - MicroRNAs control de novo DNA methylation through regulation of transcriptional repressors in mouse embryonic stem cells | Supplementary information
A mammalian microRNA cluster controls DNA methylation and telomere recombination via Rbl2-dependent regulation of DNA methyltransferases - pp268 - 279
Roberta Benetti, Susana Gonzalo, Isabel Jaco, Purificación Muñoz, Susana Gonzalez, Stefan Schoeftner, Elizabeth Murchison, Thomas Andl, Taiping Chen, Peter Klatt, En Li, Manuel Serrano, Sarah Millar, Gregory Hannon & Maria A Blasco
doi:10.1038/nsmb.1399
Abstract - | Full Text - A mammalian microRNA cluster controls DNA methylation and telomere recombination via Rbl2-dependent regulation of DNA methyltransferases | PDF (710 KB) - A mammalian microRNA cluster controls DNA methylation and telomere recombination via Rbl2-dependent regulation of DNA methyltransferases | Supplementary information
Identification of conjugation specificity determinants unmasks vestigial preference for ubiquitin within the NEDD8 E2 - pp280 - 287
Danny T Huang, Min Zhuang, Olivier Ayrault & Brenda A Schulman
doi:10.1038/nsmb.1387
Abstract - | Full Text - Identification of conjugation specificity determinants unmasks vestigial preference for ubiquitin within the NEDD8 E2 | PDF (531 KB) - Identification of conjugation specificity determinants unmasks vestigial preference for ubiquitin within the NEDD8 E2 | Supplementary information
Distinct structural elements of the adaptor ClpS are required for regulating degradation by ClpAP - pp288 - 294
Jennifer Y Hou, Robert T Sauer & Tania A Baker
doi:10.1038/nsmb.1392
Abstract - | Full Text - Distinct structural elements of the adaptor ClpS are required for regulating degradation by ClpAP | PDF (1,010 KB) - Distinct structural elements of the adaptor ClpS are required for regulating degradation by ClpAP | Supplementary information
Crystal structures of human and Staphylococcus aureus pyruvate carboxylase and molecular insights into the carboxyltransfer reaction - pp295 - 302
Song Xiang & Liang Tong
doi:10.1038/nsmb.1393
Abstract - | Full Text - Crystal structures of human and Staphylococcus aureus pyruvate carboxylase and molecular insights into the carboxyltransfer reaction | PDF (1,074 KB) - Crystal structures of human and Staphylococcus aureus pyruvate carboxylase and molecular insights into the carboxyltransfer reaction | Supplementary information
GroEL stimulates protein folding through forced unfolding - pp303 - 311
Zong Lin, Damian Madan & Hays S Rye
doi:10.1038/nsmb.1394
Abstract - | Full Text - GroEL stimulates protein folding through forced unfolding | PDF (650 KB) - GroEL stimulates protein folding through forced unfolding | Supplementary information
Binding of a neutralizing antibody to dengue virus alters the arrangement of surface glycoproteins - pp312 - 317
Shee-Mei Lok, Victor Kostyuchenko, Grant E Nybakken, Heather A Holdaway, Anthony J Battisti, Soila Sukupolvi-Petty, Dagmar Sedlak, Daved H Fremont, Paul R Chipman, John T Roehrig, Michael S Diamond, Richard J Kuhn & Michael G Rossmann
doi:10.1038/nsmb.1382
Abstract - | Full Text - Binding of a neutralizing antibody to dengue virus alters the arrangement of surface glycoproteins | PDF (807 KB) - Binding of a neutralizing antibody to dengue virus alters the arrangement of surface glycoproteins | Supplementary information
Brief Communication
HP1-mediated silencing targets Pol II coactivator complexes - pp318 - 320
Andrea Smallwood, Joshua C Black, Naoko Tanese, Sriharsa Pradhan & Michael Carey
doi:10.1038/nsmb.1385
Abstract - | Full Text - HP1-mediated silencing targets Pol II coactivator complexes | PDF (251 KB) - HP1-mediated silencing targets Pol II coactivator complexes | Supplementary information
Technical Report
In situ observation of protein phosphorylation by high-resolution NMR spectroscopy - pp321 - 329
Philipp Selenko, Dominique P Frueh, Simon J Elsaesser, Wilhelm Haas, Steven P Gygi & Gerhard Wagner
doi:10.1038/nsmb.1395
Abstract - | Full Text - In situ observation of protein phosphorylation by high-resolution NMR spectroscopy | PDF (2,253 KB) - In situ observation of protein phosphorylation by high-resolution NMR spectroscopy | Supplementary information
Corrigendum
Corrigendum: The refined structure of nascent HDL reveals a key functional domain for particle maturation and dysfunction - p330
Zhiping Wu, Matthew A Wagner, Lemin Zheng, John S Parks, Jacinto M Shy III, Jonathan D Smith, Valentin Gogonea & Stanley L Hazen
doi:10.1038/nsmb0308-330
Full Text - Corrigendum: The refined structure of nascent HDL reveals a key functional domain for particle maturation and dysfunction | PDF (347 KB) - Corrigendum: The refined structure of nascent HDL reveals a key functional domain for particle maturation and dysfunction