Brief Communication abstract
Nature Structural & Molecular Biology 15, 318 - 320 (2008)
Published online: 10 February 2008 | doi:10.1038/nsmb.1385
HP1-mediated silencing targets Pol II coactivator complexes
Andrea Smallwood1,4, Joshua C Black1,4, Naoko Tanese2, Sriharsa Pradhan3 & Michael Carey1
Little is known of the specific biochemical mechanism by which heterochromatin protein 1 (HP1) inactivates a gene. We analyzed HP1-mediated inhibition of preinitiation complex (PIC) assembly in vitro on chromatin templates regulated by GAL4-VP16 or Sp1. HP1 blocked key subunits of the TFIID and Mediator coactivator complexes. Notably, binding of the same subunits was inhibited by HP1 on the Sp1-regulated survivin gene in vivo upon DNA damage–induced silencing.
- Department of Biological Chemistry, 351A Biomedical Sciences Research Building, David Geffen School of Medicine, University of California, Los Angeles, California 90095-1737, USA.
- Department of Microbiology, 550 First Avenue, New York University School of Medicine, New York, New York 10016, USA.
- New England Biolabs, 240 County Road, Ipswich, Massachusetts 01938, USA.
- These authors contributed equally to this work.
Correspondence to: Michael Carey1 e-mail: mcarey@mednet.ucla.edu
MORE ARTICLES LIKE THIS
These links to content published by NPG are automatically generated.
NEWS AND VIEWS
The Pol II initiation complex: finding a place to startNature Structural & Molecular Biology News and Views (01 Jul 2006)
Finding the right spot to start transcriptionNature Structural & Molecular Biology News and Views (01 Aug 2007)
See all 6 matches for News And ViewsRESEARCH
CIITA coordinates multiple histone acetylation modifications at the HLA-DRA promoterNature Immunology Article (01 Jul 2001)
TAF II -independent activation mediated by human TBP in the presence of the positive cofactor PC4The EMBO Journal Article (03 Aug 1998)
See all 63 matches for Research