Supplementary information
From the following article
A torque component present in mitotic kinesin Eg5 revealed by three-dimensional tracking
Junichiro Yajima, Kana Mizutani & Takayuki Nishizaka
Nature Structural & Molecular Biology 15, 1119 - 1121 (2008) Published online: 21 September 2008
doi:10.1038/nsmb.1491
Supplementary Text and Figures
Supplementary Figures 1–4, Supplementary Methods
Supplementary Video 1
First 
1 second shows a sliding rhodamine-labeled microtubule (0.7 s intervals, 
10 actual speed, rhodamine filter set, Nikon). Remaining 9 seconds shows a moving QD 525 (Invitrogen) fixed to the microtubule via a streptavidin-biotin interaction (2.8 s intervals 300 actual speed, Qdot 525 filter set, Chroma). Image, 14 
m high 52 m wide.
Supplementary Video 2
The viewing angle is changed every 10 degrees by 360 degrees along a longitudinal axis of the microtubule. The colors (blue, sky blue, green, yellow) of the dots show the height of z-position. The blue is lowest and the yellow is highest. A distance between blue dot and yellow dot is 70 nm. See Fig. 1c
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