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The Arabidopsis LHP1 protein colocalizes with histone H3 Lys27 trimethylation

Nature Structural & Molecular Biology volume 14, pages 869–871 (2007)Cite this article

Abstract

Polycomb proteins are required for maintenance of silent chromatin states via histone H3 Lys27 trimethylation (H3K27me3) in animals, but homologs are not found in plant genomes. Using a DamID-chip method, we found that the Arabidopsis thaliana chromodomain-containing protein LHP1 colocalizes with H3K27me3 genome-wide. The LHP1 chromodomain also binds H3K27me3 with high affinity, suggesting that LHP1 has functions similar to those of Polycomb.

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Figure 1: Genome-wide Arabidopsis LHP1-binding sites.
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Acknowledgements

We apologize to authors of work uncited due to space constraints. We thank B. van Steensel (Netherlands Cancer Institute) for advice on DamID, N. Houba-Hérin for helpful discussions, I. Henderson for critical reading of the manuscript, and M. Pellegrini and S. Cokus for help with statistical analysis and data submission. X.Z. is supported by a post-doctoral fellowship from the Jonsson Cancer Center Foundation. S.G. is supported by graduate studentships from the French Ministry of Research (ACI no. 03-3-135) and from the French Cancer Research Association (ARC). Research in the Khorasanizadeh laboratory is supported by US National Institutes of Health (NIH) grant GM064786. Research in the Jacobsen laboratory is supported by NIH grant GM60398 and a grant from the NIH ENCODE Program, HG003523. S.E.J. is an investigator of the Howard Hughes Medical Institute.

Author information

Author notes

  1. Xiaoyu Zhang

    Present address: Present address: Department of Plant Biology, University of Georgia, Athens, Georgia 30602, USA.,

  2. Xiaoyu Zhang, Sophie Germann and Bartlomiej J Blus: These authors contributed equally to this work.

Authors and Affiliations

  1. Department of Molecular, Cell and Developmental Biology, University of California, Los Angeles, 90095, California, USA

    Xiaoyu Zhang & Steven E Jacobsen

  2. Laboratoire de Biologie Cellulaire, Institut J. P. Bourgin, Institut National de la Recherche Agronomique, route de St Cyr, Versailles, 78026, France

    Sophie Germann & Valerie Gaudin

  3. Department of Biochemistry and Molecular Genetics, University of Virginia Health System, Charlottesville, 22908, Virginia, USA

    Bartlomiej J Blus & Sepideh Khorasanizadeh

  4. Howard Hughes Medical Institute, University of California, Los Angeles, 90095, California, USA

    Steven E Jacobsen

Authors
  1. Xiaoyu Zhang
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  2. Sophie Germann
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  5. Valerie Gaudin
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  6. Steven E Jacobsen
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Contributions

S.K., V.G. and S.E.J. designed the experiments; X.Z., S.G. and B.J.B. performed the experiments and analyzed the data; X.Z. wrote the paper.

Corresponding authors

Correspondence to Valerie Gaudin or Steven E Jacobsen.

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The authors declare no competing financial interests.

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Supplementary Figures 1–4, Supplementary Table 1, Supplementary Methods (PDF 183 kb)

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Zhang, X., Germann, S., Blus, B. et al. The Arabidopsis LHP1 protein colocalizes with histone H3 Lys27 trimethylation. Nat Struct Mol Biol 14, 869–871 (2007). https://doi.org/10.1038/nsmb1283

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