Supplementary information

From the following article

Competitive binding of AUF1 and TIAR to MYC mRNA controls its translation

Baisong Liao, Yan Hu & Gary Brewer

Nature Structural & Molecular Biology 14, 511 - 518 (2007) Published online: 7 May 2007 Corrected online: 21 May 2007

doi:10.1038/nsmb1249

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Supplementary Fig. 1

Antibody specific for AUF1 precipitates MYC mRNA from cytoplasmic lysates of K562 cells

Supplementary Fig. 2

Effects of AUF1 knockdown on MYC gene expression in HeLa cells

Supplementary Fig. 3

Effects of AUF1 knockdown on luciferase-MYC ARE reporter gene expression in HeLa cells

Supplementary Fig. 4

TIAR antibody precipitates MYC ARE reporter mRNA but not luciferase mRNA lacking the ARE

Supplementary Fig. 5

TIAR knockdown has no effect on MYC mRNA levels in K562 cells

Supplementary Fig. 6

Effects of TIAR knockdown on luciferase–MYC ARE reporter gene expression with in K562 cells

Supplementary Fig. 7

Controls for mRNP immunoprecipitations following AUF1 and TIAR knockdown

Supplementary Fig. 8

AUF1 knockdown retards proliferation of HeLa cells

Supplementary Fig. 9

TIAR knockdown reverses the cellular proliferation defect conferred by AUF1 knockdown

Supplementary Fig. 10

MYC knockdown reverses the proliferation-inducing effects of TIAR knockdown

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