Article abstract
Nature Structural & Molecular Biology 14, 208 - 214 (2007)
Published online: 11 February 2007 | doi:10.1038/nsmb1205
RPA-like proteins mediate yeast telomere function
Hua Gao1,2, Rachel B Cervantes1, Edward K Mandell1,3, Joel H Otero3 & Victoria Lundblad1
Abstract
Cdc13, Stn1 and Ten1 are essential yeast proteins that both protect chromosome termini from unregulated resection and regulate telomere length. Cdc13, which localizes to telomeres through high-affinity binding to telomeric single-stranded DNA, has been extensively characterized, whereas the contribution(s) of the Cdc13-associated Stn1 and Ten1 proteins to telomere function have remained unclear. We show here that Stn1 and Ten1 are DNA-binding proteins with specificity for telomeric DNA substrates. Furthermore, Stn1 and Ten1 show similarities to Rpa2 and Rpa3, subunits of the heterotrimeric replication protein A (RPA) complex, which is the major single-stranded DNA–binding activity in eukaryotic cells. We propose that Cdc13, Stn1 and Ten1 function as a telomere-specific RPA-like complex. Identification of an RPA-like complex that is targeted to a specific region of the genome suggests that multiple RPA-like complexes have evolved, each making individual contributions to genomic stability.
- The Salk Institute for Biological Studies, 10010 North Torrey Pines Road, La Jolla, California 92037, USA.
- Department of Biochemistry and Molecular Biology, Baylor College of Medicine, One Baylor Plaza, Houston Texas 77030, USA.
- Graduate Program in Cell and Molecular Biology, Baylor College of Medicine, One Baylor Plaza, Houston Texas 77030, USA.
Correspondence to: Victoria Lundblad1 e-mail: lundblad@salk.edu
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