Nature Structural & Molecular Biology 13, 429 - 435 (2006)
Published online: 9 April 2006; | doi:10.1038/nsmb1080
Nucleophosmin is selectively deposited on mRNA during polyadenylationViswanathan Palaniswamy, Karen C M Moraes, Carol J Wilusz
& Jeffrey Wilusz
Department of Microbiology, Immunology & Pathology, Colorado State University, 1619 Campus Delivery, Fort Collins, Colorado 80523, USA.
Correspondence should be addressed to Jeffrey Wilusz jeffrey.wilusz@colostate.edu Nucleophosmin (NPM), an abundant, predominantly nucleolar protein that influences numerous cellular processes, was shown to specifically associate with the bodies of messenger RNAs as a result of the process of 3'-end formation. NPM deposition requires polyadenylation but not the 3' cleavage event to occur on the transcript. Furthermore, the protein does not associate with RNAs bearing a preformed poly(A) tail or with mRNAs that have undergone cleavage but not polyadenylation. A region within 10 bases upstream of the AAUAAA element is required for NPM association, but deposition of the protein seems to be sequence independent. NPM association with poly(A)+ mRNAs was also demonstrated in vivo. NPM, therefore, represents a mark left on transcripts as a result of 3'-end processing and may have a role in one or more of a variety of post-transcriptional processes influenced by the polyadenylation event.
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